Document Detail


Alterations in cell cycle genes in early stage lung adenocarcinoma identified by expression profiling.
MedLine Citation:
PMID:  12878869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In normal lung epithelial cells, cellular division is an ordered, tightly regulated process involving multiple checkpoints that assess extracellular growth signals, cell size, and DNA integrity. In contrast, neoplastic lung cells develop the ability to bypass several of these checkpoints, particularly at the G1/S and G2/M boundaries. We used genomic profiling to compare gene expression levels in early stage lung adenocarcinomas and non-neoplastic pulmonary tissue in order to comprehensively identify alterations in the process of cell cycling. RNA extracted from node negative, poorly differentiated lung adenocarcinomas (15 patients) and non-neoplastic pulmonary tissue (5 patients) was hybridized to oligonu-cleotide microarray filters containing 44,363 genes. Ontological classification was used to extract genes involved with cell cycle progression. Further analysis discovered a subset of differentially expressed genes for further study. Of the 624 cell cycle genes on the microarray filters, 40 genes were predicted to be differentially expressed in lung adeno-carcinomas. Alterations in several genes (i.e., cyclin B1, cyclin D1, p21, MDM2) are consistent with published data in the literature. We also identified 19 novel genes that have neither been described in non-small cell lung cancer (i.e., cdc2, cullin 4A, ZAC, p57, DP-1, GADD45, PISSLRE, cdc20) nor in any other tumors (i.e., cyclin F, cullin 5, p34). These results identified several potential cell cycle genes altered in lung cancer.
Authors:
Sunil Singhal; Kunjlata M Amin; Robert Kruklitis; Peter DeLong; Michael E Friscia; Leslie A Litzky; Mary E Putt; Larry R Kaiser; Steven M Albelda
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer biology & therapy     Volume:  2     ISSN:  1538-4047     ISO Abbreviation:  Cancer Biol. Ther.     Publication Date:    2003 May-Jun
Date Detail:
Created Date:  2003-07-24     Completed Date:  2003-12-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-8     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*,  metabolism
Aged
Cell Cycle / genetics
Female
Gene Expression Profiling*
Gene Expression Regulation, Neoplastic
Genes, cdc / physiology*
Humans
Lung Neoplasms / genetics*,  metabolism*
Male
Neoplasm Proteins / metabolism*
Neoplasm Staging
Oligonucleotide Array Sequence Analysis / methods
RNA, Messenger / metabolism
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
P01 66726//PHS HHS; R25 CA87812/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/RNA, Messenger
Comments/Corrections
Comment In:
Cancer Biol Ther. 2003 May-Jun;2(3):299-300   [PMID:  12878870 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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