Document Detail

Alterations of fatty acid metabolism and membrane fluidity in peroxisome-defective mutant ZP102 cells.
MedLine Citation:
PMID:  15055234     Owner:  NLM     Status:  MEDLINE    
We investigated lipid composition and FA metabolism in Chinese hamster ovary CHO-K1) cells and Pex5-mutated CHO-K1 (ZP102) cells to clarify the biochemical bases of peroxisome biogenesis disorders (PBD). ZP102 cells have defective peroxisomes and exhibit impairments of peroxisomal beta-oxidation of FA and plasmalogen biosynthesis. In addition, we identified FA metabolic alterations in the synthesis of several classes of lipids in ZP102 cells. The concentration of FFA in ZP102 cells was twice that in CHO-K1 cells, but methyl esters and TAG were decreased in ZP102 cells in comparison with control cells. Also, ceramide monohexoside (CMH) concentration with ZP102 cells was significantly increased compared with the control cells. The FA molecular species, particularly the saturated to unsaturated ratios, of individual lipids also differed between the two cell types. The rate of incorporation of [14C]-labeled saturated acids into sphingomyelin (SM) and CMH in ZP102 cells was higher than that in CHO-K1 cells. Lignoceric acid incorporated into cells was predominantly utilized for the synthesis of SM at 24 h after removal of [14C]lignoceric acid from the culture medium. ZP102 cells showed higher fluorescence anisotropy of 1,3,5-diphenylhexatriene, corresponding to lower membrane mobility than in CHO-K1 cells. In particular, alteration of lipid metabolism by a Pex5 mutation enhanced metabolism of saturated FA and sphingolipids. This may be related to the reduced membrane fluidity of ZP102 cells, which has been implicated in the dysfunction of membrane-linked processes in PBD.
Michiaki Nagura; Makiko Saito; Masao Iwamori; Yoichi Sakakihara; Takashi Igarashi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  39     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-04-01     Completed Date:  2004-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  43-50     Citation Subset:  IM    
Department of Pediatrics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
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MeSH Terms
CHO Cells
Carbon Isotopes
Fatty Acids / metabolism*
Lipid Metabolism
Membrane Fluidity*
Peroxisomes / genetics*,  metabolism
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids; 557-59-5/lignoceric acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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