Document Detail


Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material.
MedLine Citation:
PMID:  3162826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study documents the ability of substrata material derived from well but not poorly differentiated colon carcinoma cells to alter the biological characteristics of a separate colon carcinoma cell line (MOSERSF). To assess changes induced by the presence of these substrata, MOSERSF cells were screened for (a) morphological features, (b) secretion of carcinoembryonic antigen (CEA), (c) alteration of urokinase levels, and (d) sensitivity to the growth-inhibitory peptide transforming growth factor beta. Morphologically, MOSERSF cells grown on plastic displayed a rounded shape and could be detached by agitation. Subculturing of these cells onto substrata laid down by well differentiated (mature) colon carcinoma cells resulted in cell attachment and spreading. These changes did not manifest themselves when cells were plated on material derived from poorly differentiated (primitive) colon cells. Conditioned medium from MOSERSF cells grown on plastic or on colon-derived material from the well and poorly differentiated colon cells were compared for CEA levels. Substrata derived from undifferentiated cells were without effect on assayable CEA (substrata absent, 1.4 ng/ml/10(6) cells/72 h; substrata present, 1.4-1.7 ng/ml/10(6) cells/72 h). However, growth of MOSERSF cells on material deposited by well differentiated colon cells resulted in a 3-fold increase in the level of CEA. Spent medium was also analyzed for urokinase. A high level of the protease (20.3 ng/ml/10(6) cells/72 h) was expressed by MOSERSF cells. The concentration of the enzyme was reduced by over 50% when MOSERSF cells were propagated on substrata laid down by well differentiated cells. An enhanced sensitivity to the growth-retarding effects of transforming growth factor beta was seen with certain substrata. On plastic, transforming growth factor beta inhibited proliferation of MOSERSF cells with a median effective concentration of 0.65 ng/ml. However, on substrata from mature but not primitive cells, MOSERSF cells exhibited an increased sensitivity to the peptide (median effective concentration, 0.16 ng/ml). Colon-derived material obtained from both well differentiated and poorly differentiated colon carcinoma cells was compared after [35S]-methionine metabolic labeling. More [35S]methionine was incorporated into the material from the "mature" colon cells. The substrata could also be distinguished by quantitative differences in a number of high molecular weight proteins. Immunofluorescence of colon-deposited material revealed the presence of laminin and fibronectin.
Authors:
D Boyd; G Florent; S Chakrabarty; D Brattain; M G Brattain
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  48     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-03     Completed Date:  1988-06-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2825-31     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
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MeSH Terms
Descriptor/Qualifier:
Carcinoembryonic Antigen / analysis
Carcinoma / pathology*
Colonic Neoplasms / pathology*
Dimethylformamide / pharmacology
Extracellular Matrix / analysis,  physiology*
Humans
Peptides / pharmacology
Transforming Growth Factors
Tumor Cells, Cultured
Urokinase-Type Plasminogen Activator / analysis
Grant Support
ID/Acronym/Agency:
CA34432/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Carcinoembryonic Antigen; 0/Peptides; 68-12-2/Dimethylformamide; 76057-06-2/Transforming Growth Factors; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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