Document Detail

Alterations of Tamm-Horsfall protein immunoreactivity after partial desialylation and deglycosylation.
MedLine Citation:
PMID:  9017164     Owner:  NLM     Status:  MEDLINE    
The role of the carbohydrate moiety of Tamm-Horsfall protein (THP) was investigated by studying the effect of partial enzymatic desialylation and deglycosylation on its immunoreactivity. In our experiments primarily sialic acid alpha 2,6 linked to a galactose was split off, resulting in a 14% increase of immunoreactivity. It was increased up to 21% by further removal of the sugar residues. We also found that complexes composed of THP and human serum albumin, immunoglobulin G or transferrin reacted with anti-THP antibodies. When these complexes were formed with desialylated or deglycosylated THP an essential increase of this reactivity (about 25%) occurred. Our studies indicate that modifications of the carbohydrate moiety of THP (which might occur in vivo by changes in the glycosylation process e.g. in pathologic conditions) lead to increased exposure of THP to the immune system.
T Grabska; T Bagiński; A Kubicz; M Kokot; F Kokot; J Duława
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archivum immunologiae et therapiae experimentalis     Volume:  44     ISSN:  0004-069X     ISO Abbreviation:  Arch. Immunol. Ther. Exp. (Warsz.)     Publication Date:  1996  
Date Detail:
Created Date:  1997-06-03     Completed Date:  1997-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0114365     Medline TA:  Arch Immunol Ther Exp (Warsz)     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
Institute of Biochemistry, University of Wrocław, Poland.
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MeSH Terms
Antibody Specificity
Antigen-Antibody Reactions*
Carbohydrate Conformation
Carbohydrates / immunology*
Enzyme-Linked Immunosorbent Assay
Epitopes / chemistry,  immunology*
Goats / immunology
Mucoproteins / chemistry,  immunology*
N-Acetylneuraminic Acid / immunology*
Protein Processing, Post-Translational
Reg. No./Substance:
0/Carbohydrates; 0/Epitopes; 0/Mucoproteins; 0/Tamm-Horsfall protein; 131-48-6/N-Acetylneuraminic Acid

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