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Alterations of Adiponectin in the Course of Inflammation and Severe Sepsis.
MedLine Citation:
PMID:  22744305     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: The adipocyte-specific protein adiponectin reveals anti-inflammatory, anti-oxidant, anti-atherosclerotic and vaso-protective effects. This study aims to investigate adiponectin expression in cultured human adipocytes within an inflammatory model and in patients suffering from severe sepsis and evaluates treatment effects of drotrecogin alpha (activated) (DAA). METHODS: In an in-vitro inflammatory model of adipocyte cell-culture the effect of DAA on adiponectin mRNA expression was evaluated. Synthesis of mRNA was measured by quantitative polymerase chain reaction (qPCR). Supernatants of these adipocytes and serum levels of adiponectin were measured in blood of 104 patients by ELISA-method on day 1, 3 and 5 of severe sepsis. 26 patients were treated with DAA (DAA+), 78 patients without DAA (DAA-). RESULTS: Stimulation of human adipocytes with TNF alpha over 6 and 24 hours resulted in a significant decrease of adiponectin mRNA transcripts. After 24 hours of incubation adiponectin mRNA expression was significantly up-regulated according to applied dosages of DAA at 50 ng/ml and 5 μg/ml (p<0.05). Accordingly, adiponectin levels of supernatants of adipocyte culture increased after 24 hours (p<0.05). DAA- patients revealed significantly higher adiponectin serum levels compared to healthy controls (p<0.1) and DAA+ patients (p<0.05) at day 1 and 3. On day 5 after 96 hours infusion of DAA (24 μg/kg/h) adiponectin levels significantly increased in DAA+ patients and equalized towards DAA- patients (p>0.1). CONCLUSIONS: Adiponectin might be involved in the pathogenesis of the systemic inflammatory response during sepsis. Administration of DAA up-regulates adiponectin expression under circumstances of systemic inflammation.
Authors:
Michael Behnes; Martina Brueckmann; Siegfried Lang; Christian Putensen; Joachim Saur; Martin Borggrefe; Ursula Hoffmann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-27
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  -     ISSN:  1540-0514     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
From the First Department of Medicine1 (M.Be., M.Br., S.L., J.S., M.Bo., M.Br.), Faculty of Medicine Mannheim, University of Heidelberg, Mannheim, Germany and from the Department of Anesthesiology2 (C.P.), University of Bonn, Germany.
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