| Alteration of xanthine oxidase activity in sinusoidal endothelial cells and morphological changes of Kupffer cells in hypoxic and reoxygenated rat liver. | |
| | |
MedLine Citation:
|
PMID: 7768504 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the model of the perfused rat liver, we investigated the alterations of sinusoidal cells in the pathogenesis of liver injury caused by hypoxia and reperfusion. In sinusoidal endothelial cells, the activity of xanthine oxidase (XOX), a cytoplasmic marker enzyme, was located cytochemically and determined biochemically. Kupffer cells, identified by their endogenous peroxidase staining, were studied with regard to changes in their ultrastructure. In our experiments, parenchymal cells were shown to be severely damaged in contrast to sinusoidal lining cells, which showed minor signs of injury. In comparison with the control group, XOX activity increased significantly in the sinusoidal endothelial cells after low-flow hypoxia; however, after reoxygenation of only 5 minutes, that activity was lower after hypoxia but higher after control perfusion. In Kupffer cells, hypoxia resulted in a strong suppression of phagocytic and endocytotic activity and in a disappearance of the lamellopodia. Kupffer cells were flattened, resembling sinusoidal endothelial cells. After reoxygenation phagocytic vesicles, lamellopodia, and cell volume of Kupffer cells increased markedly in comparison with the control group. In the hypoxia/reperfusion injury model, our observations revealed significant alterations of sinusoidal lining cells. It appears that sinusoidal endothelial cells respond to the hypoxic phase by producing oxygen-derived free radicals and that Kupffer cells respond to the subsequent reperfusion phase by activation followed by the release of toxic mediators. |
| | |
Authors:
|
S Angermüller; M Schunk; K Kusterer |
Related Documents
:
|
175214 - Proliferation of endothelial cells in estrogen-stimulated rat liver. a light and electr... 12389664 - The protective role of kupffer cells in the ischemia-reperfused rat liver. 7089374 - In vitro cell adherence to newly excysted fasciola hepatica: failure to affect their su... 8193774 - In vitro dna synthesis as indicator of mammary epithelial cell division: [14c]thymidine... 19712044 - Development of three-dimensional biomimetic scaffold to study epithelial-mesenchymal in... 9675174 - A theoretical model study of the influence of fluid stresses on a cell adhering to a mi... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Hepatology (Baltimore, Md.) Volume: 21 ISSN: 0270-9139 ISO Abbreviation: Hepatology Publication Date: 1995 Jun |
Date Detail:
|
Created Date: 1995-06-30 Completed Date: 1995-06-30 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8302946 Medline TA: Hepatology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1594-601 Citation Subset: IM |
Affiliation:
|
Department of Anatomy and Cell Biology II, University of Heidelberg, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anoxia Cell Nucleus / ultrastructure Endothelium / enzymology, ultrastructure Female Glutamate Dehydrogenase / analysis Kupffer Cells / cytology*, pathology L-Lactate Dehydrogenase / analysis Liver / enzymology, physiology*, physiopathology Microbodies / ultrastructure Oxygen / pharmacology Rats Rats, Wistar Reperfusion Reperfusion Injury / enzymology, physiopathology Xanthine Oxidase / metabolism* |
| Chemical | |
Reg. No./Substance:
|
7782-44-7/Oxygen; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.17.3.2/Xanthine Oxidase; EC 1.4.1.2/Glutamate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protective effect of hepatocyte growth factor on interferon-gamma-induced cytotoxicity in mouse hepa...
Next Document: Monitoring of surface mitochondrial NADH levels as an indication of ischemia during liver isograft t...