| Alteration of tyrosine hydroxylase activity in PC12 cells infected with herpes simplex virus type 1. | |
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MedLine Citation:
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PMID: 2857560 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During infection with herpes simplex virus type 1 (HSV-1) the activity of tyrosine hydroxylase (TH) in PC12 pheochromocytoma cells was initially depressed reaching a nadir at 6 hours post-inoculation, but recovered rapidly with a return to baseline activity by 8 to 9 hours post-inoculation. Subsequently, TH activity again fell with a second more variable rise in activity occurring at 24 hours post-inoculation. Studies with metabolic inhibitors and 2 temperature-sensitive viral mutants indicated that these alterations of TH activity were dissociated from morphological cytopathology and likely required expression of "late" viral gene products. Immunotitration using anti-TH antibody suggested that early depression of TH activity resulted principally from loss of enzyme protein rather than simple enzyme inactivation, and that reconstitution of activity at 9 hours was related to augmented enzyme synthesis. These observations illustrate the complexity of perturbed cellular metabolism during HSV-1 infection and suggest involvement of two unexpected processes: alteration of a specialized cell function as a result of viral genes expressed late in the replicative cycle, and augmented synthesis of a cell-coded gene product during the course of infection. |
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Authors:
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R Rubenstein; R W Price; T Joh |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of virology Volume: 83 ISSN: 0304-8608 ISO Abbreviation: Arch. Virol. Publication Date: 1985 |
Date Detail:
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Created Date: 1985-03-14 Completed Date: 1985-03-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7506870 Medline TA: Arch Virol Country: AUSTRIA |
Other Details:
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Languages: eng Pagination: 65-82 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Cycloheximide / pharmacology Dactinomycin / pharmacology Gene Expression Regulation* / drug effects Humans Immunologic Techniques Pheochromocytoma Simplexvirus / physiology*, radiation effects Time Factors Tyrosine 3-Monooxygenase / metabolism* Ultraviolet Rays |
| Grant Support | |
ID/Acronym/Agency:
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NS-12396/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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50-76-0/Dactinomycin; 66-81-9/Cycloheximide; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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