| Alteration of superoxide dismutase activity in tracheal epithelial cells by asbestos and inhibition of cytotoxicity by antioxidants. | |
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MedLine Citation:
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PMID: 3945053 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report here the inhibition of asbestos-induced cytotoxicity in a hamster tracheal epithelial cell line by superoxide dismutase, a scavenger of superoxide (O2-.), and by mannitol and dimethylthiourea, scavengers of the hydroxyl radical (OH.). By using these agents, cell damage was ameliorated in cultures exposed to long (greater than 10 microns in length) fibers of chrysotile and crocidolite asbestos. In contrast, injury to epithelial cells by short (less than or equal to 2 microns) chrysotile or glass fibers was not prevented by scavengers of O2-., OH., H2O2 or 1O2 (singlet oxygen). These results implicate active oxygen species as mediators of injury by long asbestos fibers to cells of the respiratory tract. By using immunocytochemical and biochemical techniques, we detected appreciable amounts of copper-zinc superoxide dismutase in hamster tracheobronchial epithelial cells and alveolar macrophages in vitro and in histologic sections of rat and human respiratory tract. Activity of total endogenous superoxide dismutase (copper-zinc and manganese forms) increased in tracheal epithelial cells exposed for several days in vitro to either crocidolite or chrysotile asbestos but was unchanged in untreated cells and those exposed to comparable amounts of glass fibers. After inhalation of asbestos by rats, or exposure of cells in culture to asbestos, long fibers were observed protruding from both epithelial cells and alveolar macrophages. The unsuccessful phagocytosis of long fibers of asbestos coupled with generation of oxygen free radicals might explain the increased pathogenic potential of long fibers in asbestos-associated diseases of the respiratory tract. |
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Authors:
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B T Mossman; J P Marsh; M A Shatos |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 54 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1986 Feb |
Date Detail:
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Created Date: 1986-03-26 Completed Date: 1986-03-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 204-12 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology* Asbestos / antagonists & inhibitors, pharmacology* Clone Cells Cricetinae Epithelial Cells Epithelium / drug effects, enzymology Free Radicals Histocytochemistry Immunochemistry Microscopy, Electron Minerals / pharmacology Oxygen / metabolism Radioisotopes / diagnostic use Selenium / diagnostic use, metabolism Superoxide Dismutase / metabolism* Trachea / cytology, drug effects, enzymology* |
| Grant Support | |
ID/Acronym/Agency:
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14212//PHS HHS; R01 33501//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Free Radicals; 0/Minerals; 0/Radioisotopes; 1332-21-4/Asbestos; 7782-44-7/Oxygen; 7782-49-2/Selenium; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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