| Alteration of the spermatozoal glycocalyx and its effect on duration of fertility in the fowl (Gallus domesticus). | |
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MedLine Citation:
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PMID: 2547463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypothesis that sialic acid has a role in spermatozoal sequestration within the hen's oviduct was tested by treating spermatozoa with Clostridium perfringens neuraminidase. Spermatozoal content of sialic acid ranged from 94 to 135 micrograms per 10(9) spermatozoa (n = 12 roosters). Spermatozoa contained 80% of total seminal sialic acid (coefficient of variation = 4.6%). Spermatozoal sialic acid content was reduced by 18% when 10(9) spermatozoa were incubated at pH 6.5 with 10 IU neuraminidase activity (Type V, Sigma Chemical Co.). Such treatment had no effect on spermatozoal viability as evidenced by ethidium bromide uptake. However, treatment of spermatozoa with neuraminidase prior to intravaginal insemination reduced fertility by 24 percentage units (p less than 0.001). In contrast, when similarly treated spermatozoa were deposited in the magnum via laparatomy, fertility was not affected (p greater than 0.05). The preceding work was done with neuraminidase prepared by salt fractionation (Type V, Sigma Chemical Co.). Type V neuraminidase was absorbed to diethylaminoethyl-Sephacel and then eluted with a stepwise KCl gradient. Treatment of spermatozoa with this preparation of neuraminidase (10 IU/10(9) spermatozoa) prior to intravaginal insemination reduced fertility by 19 percentage units (p less than 0.001). Decreased fertility could not be attributed to contamination of neuraminidase preparation with proteolytic activity. We conclude that spermatozoal sialic acid has a role in spermatozoal sequestration within the hen's utero-vaginal glands. |
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Authors:
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D P Froman; H N Engel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of reproduction Volume: 40 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1989 Mar |
Date Detail:
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Created Date: 1989-09-11 Completed Date: 1989-09-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 615-21 Citation Subset: IM |
Affiliation:
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Department of Poultry Science, Oregon State University, Corvallis 97331. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Clostridium perfringens / enzymology Female Fertilization* Glycoproteins / metabolism* Insemination, Artificial Male N-Acetylneuraminic Acid Neuraminidase / metabolism Oviducts / analysis* Polysaccharides / metabolism* Sialic Acids / analysis*, physiology Sperm-Ovum Interactions Spermatozoa / analysis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Polysaccharides; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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