| Alteration of protein kinase C activity after transient focal cerebral ischemia in mice using in vitro [3H]phorbol-12,13-dibutyrate binding autoradiography. | |
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MedLine Citation:
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PMID: 9452194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Changes in the regional distribution of protein kinase C (PKC) after transient focal cerebral ischemia in SV-129 mice were assessed by quantitative autoradiography using [3H]phorbol-12,13-dibutyrate ([3H]PDBu) binding. [3H]PDBu binding did not change up to 10 min after reperfusion of 3 h ischemia, but at 1 h after reperfusion markedly decreased to 40-50% of control (pre-ischemia) in the ipsilateral striatum and the middle cerebral artery (MCA) region of cortex in SV-129 mice. The binding decreased to 20% of control at 3-7 days after reperfusion, but did not change in the ipsilateral anterior cerebral artery (ACA) territory or the contralateral brain. In the ipsilateral substantia nigra, which lies outside the ischemic zone, [3H]PDBu binding was not significantly changed compared to the control values (pre-ischemia) at early phase (up to 3 h after reperfusion), but marked reduction of the binding was observed 1 day after reperfusion. After 3 h ischemia followed by 3 h reperfusion, the morphological damage and the decrease in [3H]PDBu binding in the ipsilateral striatum and the MCA region of cortex was smaller in mice lacking the expression of neuronal nitric oxide synthase (type I NOS) gene mutant mice compared to wild-type (SV-129 and C57black/6) mice. Our data suggest that postischemic alterations of PKC binding activity were observed in the ischemic and non-ischemic lesions in the mouse brain. |
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Authors:
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H Hara; G Ayata; P L Huang; M A Moskowitz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 774 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1997 Nov |
Date Detail:
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Created Date: 1998-03-11 Completed Date: 1998-03-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 69-76 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery and Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA. t86065@osa.isd.kanebo.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Brain / metabolism, pathology Female Ischemic Attack, Transient / metabolism*, pathology Male Mice Mice, Inbred C57BL Mice, Inbred Strains / metabolism Mice, Mutant Strains Phorbol 12,13-Dibutyrate / metabolism* Protein Kinase C / metabolism* Reperfusion Time Factors Tissue Distribution Tritium |
| Grant Support | |
ID/Acronym/Agency:
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NS10828/NS/NINDS NIH HHS; NS2683/NS/NINDS NIH HHS; NS33335/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 37558-16-0/Phorbol 12,13-Dibutyrate; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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