| Alteration of mitochondrial function in a model of chronic ischemia in vivo in rat heart. | |
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MedLine Citation:
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PMID: 11834475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate mitochondrial alterations in an animal model of chronic myocardial ischemia in rats obtained by surgical constriction of the left coronary artery. Resting coronary blood flow was measured using the fluorescent microsphere technique. Contractile function, defined by rate-pressure product, and myocardial oxygen consumption were measured in a Langendorff preparation. The mitochondrial function was evaluated on permeabilized skinned fibers. Three weeks after surgery, ischemic hearts showed a significant decrease in coronary blood flow compared with sham. Hemodynamic measurements showed a significant systolic and diastolic dysfunction. Alterations in mitochondrial function in ischemic hearts were mainly characterized by a significant decrease in the maximal velocity and apparent half-saturation constant for ADP, loss of the stimulatory effect of creatine, and a stimulatory effect of exogenous cytochrome c. These functional alterations were supported by structural alterations characterized by mitochondrial clustering and swelling associated with membrane rupture. We conclude that the alterations in systolic function after chronic ischemia are supported by severe modifications of mitochondrial structure and function. |
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Authors:
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Sihem Boudina; Muriel N Laclau; Liliane Tariosse; Danièle Daret; Gérard Gouverneur; Simone Bonoron-Adèle; Valdur A Saks; Pierre Dos Santos |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 282 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-02-08 Completed Date: 2002-04-03 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H821-31 Citation Subset: IM |
Affiliation:
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Institut National de la Santé et de la Recherche Médicale U441, Athérosclérose and Institut Fédératif de Recherche 4, 33600 Pessac, Estonia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Animals Calcium / metabolism Coronary Circulation Coronary Vessels / physiology Creatine / metabolism Cytochrome c Group / metabolism Energy Metabolism Hemodynamics / physiology* Mitochondria, Heart / metabolism*, ultrastructure Models, Cardiovascular Myocardial Contraction Myocardial Ischemia / physiopathology* Myocardium / ultrastructure Oxygen Consumption Permeability Rats |
| Chemical | |
Reg. No./Substance:
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0/Cytochrome c Group; 57-00-1/Creatine; 58-64-0/Adenosine Diphosphate; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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