Document Detail


Alteration of microRNA profiles in squamous cell carcinoma of the head and neck cell lines by human papillomavirus.
MedLine Citation:
PMID:  20652977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Human papillomavirus (HPV)-positive cases of squamous cell carcinoma of the head and neck (SCCHN) have a much better disease outcome compared to SCCHN cases lacking HPV. Differences in microRNA (miRNA) expression may affect their clinical outcomes.
METHODS: The miRNA expression was studied using microarrays and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in HPV-16-positive and HPV-negative SCCHN cell lines. The role of HPV-16 E6 and E7 oncogenes in altering miRNA expression was investigated using human foreskin keratinocytes (HFKs).
RESULTS: The miRNAs miR-363, miR-33, and miR-497 were upregulated, whereas miR-155, miR-181a, miR-181b, miR-29a, miR-218, miR-222, miR-221, and miR-142-5p were downregulated in HPV-positive cells compared to both HPV-negative SCCHN and normal oral keratinocytes. HPV-16 E6 oncogene altered miRNA expression in HFKs and in an HPV-16-positive cell line with E6 knockdown using siRNA.
CONCLUSION: miRNAs differentially expressed in the presence of HPV-16 may provide biomarkers for SCCHN and identify cellular pathways targeted by this virus.
Authors:
Abigail I Wald; Elizabeth E Hoskins; Susanne I Wells; Robert L Ferris; Saleem A Khan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Head & neck     Volume:  33     ISSN:  1097-0347     ISO Abbreviation:  Head Neck     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-22     Completed Date:  2011-08-03     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8902541     Medline TA:  Head Neck     Country:  United States    
Other Details:
Languages:  eng     Pagination:  504-12     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley Periodicals, Inc.
Affiliation:
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA. khan@pitt.edu
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / genetics,  metabolism,  virology*
Cell Line, Tumor
Down-Regulation
Head and Neck Neoplasms / genetics,  metabolism,  virology*
Human papillomavirus 16*
Humans
Keratinocytes / metabolism
MicroRNAs / metabolism*
Papillomavirus Infections / complications
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
2T32 AI049820/AI/NIAID NIH HHS; R01 DE016406/DE/NIDCR NIH HHS; R01 DE016406-05/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/MicroRNAs
Comments/Corrections

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