| Alteration of microRNA profiles in squamous cell carcinoma of the head and neck cell lines by human papillomavirus. | |
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MedLine Citation:
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PMID: 20652977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Human papillomavirus (HPV)-positive cases of squamous cell carcinoma of the head and neck (SCCHN) have a much better disease outcome compared to SCCHN cases lacking HPV. Differences in microRNA (miRNA) expression may affect their clinical outcomes. METHODS: The miRNA expression was studied using microarrays and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in HPV-16-positive and HPV-negative SCCHN cell lines. The role of HPV-16 E6 and E7 oncogenes in altering miRNA expression was investigated using human foreskin keratinocytes (HFKs). RESULTS: The miRNAs miR-363, miR-33, and miR-497 were upregulated, whereas miR-155, miR-181a, miR-181b, miR-29a, miR-218, miR-222, miR-221, and miR-142-5p were downregulated in HPV-positive cells compared to both HPV-negative SCCHN and normal oral keratinocytes. HPV-16 E6 oncogene altered miRNA expression in HFKs and in an HPV-16-positive cell line with E6 knockdown using siRNA. CONCLUSION: miRNAs differentially expressed in the presence of HPV-16 may provide biomarkers for SCCHN and identify cellular pathways targeted by this virus. |
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Authors:
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Abigail I Wald; Elizabeth E Hoskins; Susanne I Wells; Robert L Ferris; Saleem A Khan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Head & neck Volume: 33 ISSN: 1097-0347 ISO Abbreviation: Head Neck Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-22 Completed Date: 2011-08-03 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 8902541 Medline TA: Head Neck Country: United States |
Other Details:
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Languages: eng Pagination: 504-12 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Wiley Periodicals, Inc. |
Affiliation:
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Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA. khan@pitt.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Squamous Cell
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genetics,
metabolism,
virology* Cell Line, Tumor Down-Regulation Head and Neck Neoplasms / genetics, metabolism, virology* Human papillomavirus 16* Humans Keratinocytes / metabolism MicroRNAs / metabolism* Papillomavirus Infections / complications Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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2T32 AI049820/AI/NIAID NIH HHS; R01 DE016406/DE/NIDCR NIH HHS; R01 DE016406-05/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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