| Alteration of membrane-associated enzymes in drug-resistant sublines of mouse lymphoblastoma L5178Y cells. | |
| | |
MedLine Citation:
|
PMID: 6173369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Activities of marker enzymes for various cell components were studied with extracts of adriamycin-, aclacinomycin A- and bleomycin-resistant cells and with partially purified plasma membrane fraction of aclacinomycin A-resistant cells, in comparison with those of the parental cells. Alkaline phosphodiesterase and Na+-K+-ATPase activities were observed to alter in the drug-resistant sublines, but other enzymes showed similar activities in the resistant cells to those in the parental cells. Alkaline phosphodiesterase activities in all the resistant sublines were higher than that in the parental cells. Na+K+-ATPase activities of anthracycline-resistant sublines were lower and that in bleomycin-resistant cell line was higher than that of the parental cells. The adriamycin-resistant cells exhibited the same level of alkaline phosphodiesterase activity with the aclacinomycin A-resistant cells: Vmax was the same with, and the affinity was twice stronger than the parental cells. The bleomycin-resistant cells showed ca. 30% Vmax in comparison with the sensitive cells, and 17 fold higher affinity than the parental cells. The current results, concerning changes of membrane-associated enzymes in drug-resistant sublines of L5178Y cells, support the assumption that the resistance is due to alteration of plasma membrane transport systems. |
| | |
Authors:
|
Y Sugimoto; T Nishimura; H Suzuki; N Tanaka |
Related Documents
:
|
8548339 - Development of zidovudine (azt) resistance in jurkat t cells is associated with decreas... 20331629 - Quantitative analysis of cisplatin sensitivity of human esophageal squamous cancer cell... 6734569 - Cadmium uptake and metabolism in cultured cells. 19911269 - Role of gpr30 in the mechanisms of tamoxifen resistance in breast cancer mcf-7 cells. 19943199 - Autophagy inhibition in combination cancer treatment. 19843859 - Deletion of pten promotes tumorigenic signaling, resistance to anoikis, and altered res... 21118609 - Intramammary inoculation of panax ginseng extract in cows at drying off enhances early ... 11180409 - Cell-cycle arrest in trka-expressing nih3t3 cells involves nitric oxide synthase. 15140969 - Igfbp-3, a marker of cellular senescence, is overexpressed in human papillomavirus-immo... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of antibiotics Volume: 34 ISSN: 0021-8820 ISO Abbreviation: J. Antibiot. Publication Date: 1981 Sep |
Date Detail:
|
Created Date: 1982-04-12 Completed Date: 1982-04-12 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0151115 Medline TA: J Antibiot (Tokyo) Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 1200-5 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aclarubicin Animals Antibiotics, Antineoplastic / metabolism Bleomycin / pharmacology Cell Line Cell Membrane / enzymology Doxorubicin / pharmacology Drug Resistance Lymphoma, Non-Hodgkin / drug therapy, enzymology* Mice Naphthacenes / pharmacology Phosphoric Diester Hydrolases / analysis Sarcoma, Experimental / drug therapy, enzymology Sodium-Potassium-Exchanging ATPase / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Antibiotics, Antineoplastic; 0/Naphthacenes; 11056-06-7/Bleomycin; 23214-92-8/Doxorubicin; 57576-44-0/Aclarubicin; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mechanism of protection of protein synthesis against streptomycin inhibition in a producing strain.
Next Document: Transposition of a chromosomal segment bounded by redundant rRNA genes into other rRNA genes in Esch...