| Alteration of lymphocyte membrane phospholipids and intracellular free calcium concentrations in hyperlipidemic subjects. | |
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MedLine Citation:
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PMID: 9125292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypercholesterolemia has been proposed to influence cell functions via changes in membrane composition. The aim of the present study was to determine whether the membrane phospholipid composition of human lymphocytes is modified in hypercholesterolemia and whether these changes are accompanied by functional modifications. The phospholipid fatty acid contents and intracellular free calcium concentrations were determined in peripheral blood lymphocytes from 13 subjects with serum total cholesterol levels ranging from 4.6 to 8.8 mmol/l. The spontaneous basal rate of thymidine incorporation in lymphocyte of hypercholesterolemic individuals increased, while its relative stimulation by ConA was less effective. Important changes in membrane lipid composition, consisting mainly of decrease of the mass of phospholipids, and of associated polyunsaturated fatty acids were observed in hypercholesterolemia. In contrast, the cell cholesterol content was significantly increased. The intracellular free calcium concentration was enhanced and strongly associated with circulating cholesterol levels, cell cholesterol content and phospholipid fatty acids. These results indicate that hypercholesterolemia is accompanied by profound changes in lymphocyte membrane lipid composition and Ca(2+) handling. |
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Authors:
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I Seres; M Freyss-Béguin; A Mohácsi; B Kozlovsky; J Simon; M A Devynck; T Fülöp |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Atherosclerosis Volume: 121 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1997-04-18 Completed Date: 1997-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: IRELAND |
Other Details:
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Languages: eng Pagination: 175-83 Citation Subset: IM |
Affiliation:
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First Department of Medicine, University Medical School of Debrecen, Hungary. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism* Cholesterol / metabolism Fatty Acids / metabolism Female Humans Hypercholesterolemia / metabolism* Intracellular Fluid / metabolism* Lymphocytes / metabolism* Male Membrane Lipids / metabolism* Middle Aged Phospholipids / metabolism* Spectrometry, Fluorescence Thymidine / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Membrane Lipids; 0/Phospholipids; 50-89-5/Thymidine; 57-88-5/Cholesterol; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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