Document Detail

Alteration in the regulation of plasma membrane glycoproteins of the hepatocyte during ontogeny.
MedLine Citation:
PMID:  1969354     Owner:  NLM     Status:  MEDLINE    
The expression of four integral membrane glycoproteins was examined in detail utilizing monospecific antibodies during liver development. These included asialoglycoprotein receptor, a hepatocyte glycoprotein residing in the sinusoidal domain, and three bile canalicular glycoproteins, leucine aminopeptidase, dipeptidyl peptidase IV, and a Mr 110,000 glycoprotein denoted GP 110. It was observed that asialoglycoprotein receptor, GP 110, and dipeptidyl peptidase IV were present in low amounts in fetal liver and reached adult levels between 1 to 3 weeks. In contrast, leucine aminopeptidase was present in nearly adult amounts in 18-day-old fetal livers. These observations were qualitatively confirmed by indirect immunofluorescent staining of frozen thin liver sections obtained from fetal and adult rats. Further, in fetal livers it was found that leucine aminopeptidase was not localized to typical bile canalicular areas. Immunoprecipitation studies performed in the presence of proteolytic inhibitors using detergent-solubilized extracts of metabolically labeled liver minces revealed that GP 110 was present in low amounts as Mr 110,000 and Mr 105,000 polypeptides in 17-day fetal livers but by 21 days of gestation the larger polypeptide was the major synthesis product. Conversely, the apparent molecular weights of leucine aminopeptidase and dipeptidyl peptidase IV were not altered during development. Experiments determining relative rates of synthesis using excess amounts of antibodies showed that the concentrations of the three bile canalicular glycoproteins in liver during ontogeny reflect their rates of synthesis. These results underscore that plasma membrane constituents of the hepatocyte undergo dramatic changes in expression and localization as the liver changes its physiological role at birth.
J K Petell; A Quaroni; W J Hong; D C Hixson; S Amarri; S Reif; Y Bujanover
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  187     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-07     Completed Date:  1990-05-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  299-308     Citation Subset:  IM    
Colucci Memorial Liver Research Center, Children's Hospital, Buffalo, New York.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Antigens, CD26
Asialoglycoprotein Receptor
Cell Membrane / ultrastructure
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / analysis
Fluorescent Antibody Technique
Immune Sera
Immunoglobulin G
Leucyl Aminopeptidase / analysis
Liver / embryology,  enzymology,  growth & development*
Membrane Glycoproteins / analysis,  biosynthesis*
Molecular Weight
Rats, Inbred BUF
Receptors, Immunologic / analysis
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Asialoglycoprotein Receptor; 0/Immune Sera; 0/Immunoglobulin G; 0/Membrane Glycoproteins; 0/Receptors, Immunologic; EC Aminopeptidase; EC 3.4.14.-/Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; EC, CD26

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