Document Detail


Alteration in the regulation of plasma membrane glycoproteins of the hepatocyte during ontogeny.
MedLine Citation:
PMID:  1969354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of four integral membrane glycoproteins was examined in detail utilizing monospecific antibodies during liver development. These included asialoglycoprotein receptor, a hepatocyte glycoprotein residing in the sinusoidal domain, and three bile canalicular glycoproteins, leucine aminopeptidase, dipeptidyl peptidase IV, and a Mr 110,000 glycoprotein denoted GP 110. It was observed that asialoglycoprotein receptor, GP 110, and dipeptidyl peptidase IV were present in low amounts in fetal liver and reached adult levels between 1 to 3 weeks. In contrast, leucine aminopeptidase was present in nearly adult amounts in 18-day-old fetal livers. These observations were qualitatively confirmed by indirect immunofluorescent staining of frozen thin liver sections obtained from fetal and adult rats. Further, in fetal livers it was found that leucine aminopeptidase was not localized to typical bile canalicular areas. Immunoprecipitation studies performed in the presence of proteolytic inhibitors using detergent-solubilized extracts of metabolically labeled liver minces revealed that GP 110 was present in low amounts as Mr 110,000 and Mr 105,000 polypeptides in 17-day fetal livers but by 21 days of gestation the larger polypeptide was the major synthesis product. Conversely, the apparent molecular weights of leucine aminopeptidase and dipeptidyl peptidase IV were not altered during development. Experiments determining relative rates of synthesis using excess amounts of antibodies showed that the concentrations of the three bile canalicular glycoproteins in liver during ontogeny reflect their rates of synthesis. These results underscore that plasma membrane constituents of the hepatocyte undergo dramatic changes in expression and localization as the liver changes its physiological role at birth.
Authors:
J K Petell; A Quaroni; W J Hong; D C Hixson; S Amarri; S Reif; Y Bujanover
Related Documents :
24714634 - Prevention of cpg-induced pregnancy disruption by adoptive transfer of in vitro-induced...
23644584 - Experimental methods for testing the effects of neurotrophic peptide, adnf-9, against a...
22578624 - Minimally invasive transabdominal collection of preimplantation embryos from the common...
8469484 - Acute fatty liver of pregnancy in a patient with chronic active hepatitis and associate...
9107444 - Similar pregnancy and spontaneous abortion rates after treatment with low-dose human me...
22136754 - Pregnancy outcomes in adolescents in the uk and ireland growing up with hiv.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  187     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-07     Completed Date:  1990-05-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  299-308     Citation Subset:  IM    
Affiliation:
Colucci Memorial Liver Research Center, Children's Hospital, Buffalo, New York.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Antibodies, Monoclonal / diagnostic use
Antigens, CD26
Asialoglycoprotein Receptor
Cell Membrane / ultrastructure
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / analysis
Fetus
Fluorescent Antibody Technique
Homeostasis
Immune Sera
Immunoglobulin G
Leucyl Aminopeptidase / analysis
Liver / embryology,  enzymology,  growth & development*
Membrane Glycoproteins / analysis,  biosynthesis*
Molecular Weight
Rats
Rats, Inbred BUF
Receptors, Immunologic / analysis
Grant Support
ID/Acronym/Agency:
AM 32656/AM/NIADDK NIH HHS; CA 38773/CA/NCI NIH HHS; GM 34032/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Asialoglycoprotein Receptor; 0/Immune Sera; 0/Immunoglobulin G; 0/Membrane Glycoproteins; 0/Receptors, Immunologic; EC 3.4.11.1/Leucyl Aminopeptidase; EC 3.4.14.-/Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; EC 3.4.14.5/Antigens, CD26

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of pertussis toxin on inhibition of synaptosomal tyrosine hydroxylase activity by apomorphin...
Next Document:  Early fetal acquisition of the chromaffin and neuronal immunophenotype by human adrenal medullary ce...