Document Detail


Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training.
MedLine Citation:
PMID:  22402934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aims of this study were twofold: (1) to identify whether peripheral artery disease (PAD) patients had increased muscle concentration of angiogenic VEGF-A, anti-angiogenic VEGF₁₆₅b or VEGF receptor 1 (VEGF-R1) when compared with control subjects, and (2) to evaluate whether exercise training in PAD patients was associated with changes in muscle concentration of VEGF-A, VEGF₁₆₅b or VEGF-R1. At baseline, 22 PAD and 30 control subjects underwent gastrocnemius muscle biopsy. Twelve PAD patients were treated with supervised exercise training (SET) and underwent muscle biopsy after 3 weeks and 12 weeks of training and had sufficient tissue to measure VEGF-A, VEGF₁₆₅b and VEGF-R1 concentrations in skeletal muscle lysates by ELISA. Muscle concentrations of VEGF-A and VEGF₁₆₅b were similar in PAD patients versus controls at baseline. At both time points after the start of SET, VEGF-A levels decreased and there was a trend towards increased VEGF₁₆₅b concentrations. At baseline, VEGF-R1 concentrations were lower in PAD patients when compared with controls but did not change after SET. Skeletal muscle concentrations of VEGF-A are not different in PAD patients when compared with controls at baseline. SET is associated with a significant reduction in VEGF-A levels and a trend towards increased VEGF₁₆₅b levels. These somewhat unexpected findings suggest that further investigation into the mechanism of vascular responses to exercise training in PAD patients is warranted.
Authors:
W Schuyler Jones; Brian D Duscha; Jennifer L Robbins; Natasha N Duggan; Judith G Regensteiner; William E Kraus; William R Hiatt; Ayotunde O Dokun; Brian H Annex
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Publication Detail:
Type:  Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-08
Journal Detail:
Title:  Vascular medicine (London, England)     Volume:  17     ISSN:  1477-0377     ISO Abbreviation:  Vasc Med     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-12     Completed Date:  2012-08-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9610930     Medline TA:  Vasc Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  94-100     Citation Subset:  IM    
Affiliation:
Division of Cardiology, Duke University Medical Center, Durham, NC, USA. schuyler.jones@duke.edu
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MeSH Terms
Descriptor/Qualifier:
Aged
Analysis of Variance
Biopsy
Capillaries / physiopathology
Colorado
Enzyme-Linked Immunosorbent Assay
Exercise Therapy*
Exercise Tolerance
Female
Humans
Intermittent Claudication / etiology,  metabolism,  physiopathology,  therapy*
Male
Middle Aged
Muscle, Skeletal / blood supply,  metabolism*
Neovascularization, Physiologic*
North Carolina
Peripheral Arterial Disease / complications,  metabolism,  physiopathology,  therapy*
Recovery of Function
Time Factors
Treatment Outcome
Vascular Endothelial Growth Factor A / metabolism*
Vascular Endothelial Growth Factor Receptor-1 / metabolism
Grant Support
ID/Acronym/Agency:
1R01 HL101200S1/HL/NHLBI NIH HHS; R01 HL075752/HL/NHLBI NIH HHS; R01 HL101200/HL/NHLBI NIH HHS; R01 HL101200/HL/NHLBI NIH HHS; R01 HL75752/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; EC 2.7.10.1/FLT1 protein, human; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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