| Alteration of fatty acid composition of LM cells by lipid supplementation and temperature. | |
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MedLine Citation:
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PMID: 1167335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alteration of the fatty acid composition of monolayer cultures of LM cells grown in chemically defined medium was achieved by supplementation with fatty acids complexed to bovine serum albumin. Phospholipids containing up to 40% linoleate were found in cells grown in medium containing 20 mu g of linoleate/ml. Incorporation of linoleate into phospholipids reached a plateau after 12-24 hr, and cells remained viable for at least 3-4 days. Although linoleic, linolenic, and arachidonic acids were incorporated into LM cells equally well, only the latter was elongated by these cells under these experimental conditions. Nonadecanoic acid was incorporated to a lesser extent than the polyunsaturated fatty acids. Phosphatidylcholine and phosphatidylethanolamine of LM cells had different fatty acid compositions; phosphatidylethanolamine contained more longer chain and unsaturated fatty acids. Cells were also grown in the absence of choline and presence of choline analogs such as N,N-dimethylethanolamine, N-methylethanolamine, 3-amino-1-propanol, and 1-2-amino-1-butanol. The analog phospholipids in these cells had fatty acid compositions which were intermediate between those of phosphatidylethanolamine and phosphatidylcholine of control cells grown in the presence of choline. Linoleate was found in both phosphatidylcholine and phosphatidylethanolamine of cells supplemented with linoleate. The sphingolipid fraction of these cells, however, did not contain significant amounts of linoleate. When linoleate was present in the phospholipids, compensatory decreases in the oleate and palmitoleate content of phospholipids were observed. Lowering of the growth temperature to 28 degrees produced an increase in unsaturate fatty acid content of the phospholipids. When linoleate was supplied to cells grown at 28 degrees, there was no further increase in the unsaturated fatty acid composition of the phospholipids. Using both fatty acid supplementation and lowered growth temperature, LM cell membranes can be produced which have phospholipids with vastly different fatty acid compositions. |
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Authors:
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K A Ferguson; M Glaser; W H Bayer; P R Vagelos |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 14 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1975 Jan |
Date Detail:
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Created Date: 1975-04-21 Completed Date: 1975-04-21 Revised Date: 2007-03-19 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 146-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acids / metabolism Cattle Cell Line Fatty Acids / metabolism* Fatty Acids, Nonesterified / pharmacology* Fibroblasts / metabolism* Linoleic Acids / metabolism Linolenic Acids / metabolism Mice Phospholipids / biosynthesis Serum Albumin, Bovine Temperature* |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Linoleic Acids; 0/Linolenic Acids; 0/Phospholipids; 0/Serum Albumin, Bovine; 0/nonadecanoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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