Document Detail

Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens.
MedLine Citation:
PMID:  11752708     Owner:  NLM     Status:  MEDLINE    
Reverse genetics technology offers the possibility to study the influence of particular amino acids of infectious bursal disease virus (IBDV) on adaptation to tissue culture. Genomic segments A and B of the very virulent (vv) IBDV field strain UK661 were completely cloned and sequenced, and the strain was rescued from full-length cDNA copies of both segments (UK661rev). Using site-directed mutagenesis, alteration of a single amino acid in the segment A-encoded VP2 (A284T) resulted in a limited capacity of UK661 to replicate in tissue culture. Additional alteration of a second amino acid (Q253H) increased replication efficiency in tissue culture. The second mutant (UK661-Q253H-A284T) was used to infect chickens and results were compared with UK661 and UK661rev. Whereas UK661 and UK661rev induced 100% morbidity and 50-80% mortality, UK661-Q253H-A284T proved to be strikingly attenuated, producing neither morbidity nor mortality. Moreover, UK661-Q253H-A284T-infected animals were protected from challenge infection. Thus, alteration of two specific amino acids in the VP2 region of IBDV resulted in tissue culture adaptation and attenuation in chickens of vvIBDV. The data demonstrate that VP2 plays a decisive role in pathogenicity of IBDV.
A A W M van Loon; N de Haas; I Zeyda; E Mundt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  83     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2002-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  121-9     Citation Subset:  IM    
Intervet International BV, PO Box 31, 5830 AA Boxmeer, The Netherlands.
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MeSH Terms
Adaptation, Physiological / genetics*,  physiology
Amino Acid Substitution
Antigens, Viral / analysis
Base Sequence
Birnaviridae Infections / immunology,  virology*
Culture Techniques
DNA, Viral
Infectious bursal disease virus / genetics,  immunology,  physiology*
Molecular Sequence Data
Sequence Homology, Nucleic Acid
Viral Structural Proteins / genetics*,  physiology
Virus Replication
Reg. No./Substance:
0/Antigens, Viral; 0/DNA, Viral; 0/VP2 protein, infectious bursal disease virus; 0/Viral Structural Proteins

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