Document Detail


Alteration of 6-phosphofructo-1-kinase isozyme pools during heart development and aging.
MedLine Citation:
PMID:  2946693     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nature of 6-phosphofructo-1-kinase isozyme pools in fetal, neonatal, young adult (3 months), and aged (30 months) rat hearts was studied using chromatographic and immunological techniques. Furthermore, the changing subunit composition of each isozyme pool was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis on 6% slab gels and by immunoblotting with subunit-specific antibodies. Although all three subunit types were expressed in heart throughout life, total activity and the nature of the isozyme pools varied during neonatal development and in aged heart. In fetal heart, the complex tetramers containing all three subunits appeared to be the major isozyme types. As the heart matured to the young adult stage, the M-type subunit increased over 6-fold; whereas the changes in the other two subunits were considerably less. These data indicate that during neonatal heart maturation the isozymic pools progressively exhibited increased amounts of the tetrameric forms containing two or more M-type subunits. In aged heart relative to the young adult (3 months) heart, the total activity and proportion of M-type subunit in the isozymes were decreased; and consequently, the amounts of the M-rich isozymes were decreased. The shifts in the types of isozymes during heart maturation and subsequent aging were primarily due to changes in availability of the M-type subunit to participate in random assembly of the tetrameric isozymes.
Authors:
G A Dunaway; T P Kasten; P Kolm
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  261     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-01-22     Completed Date:  1987-01-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17170-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Animals, Newborn
Brain / enzymology
Fetus
Heart / growth & development*
Isoenzymes / metabolism*
Myocardium / enzymology*
Phosphofructokinase-1 / metabolism*
Rats
Rats, Inbred F344
Rats, Inbred Strains
Species Specificity
Grant Support
ID/Acronym/Agency:
HD 16666/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 2.7.1.11/Phosphofructokinase-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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