| Alphafetoprotein is not a component of the 8 S estradiol receptor from the immature mouse uterus or brain. | |
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MedLine Citation:
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PMID: 77069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypothesis that alphafetoprotein (AFP) could be a consitituent of the estradiol receptor in the uterus and brain of the immature mouse has been tested. Cytosols prepared from 2 week-old mice were depleted of AFP by immunoadsorption with a Sepharose-coupled anti-mouse AFP anti-serum. Aliquots of these cytosols containing nondetectable levels of AFP by RIA were then brought to 0.4 M KCl to convert 8 S estradiol receptors to the 4-5 S forms, reacted either with anti-AFP or with a control anti-IgG immunoadsorbent, labeled with 3H-estradiol, and centrifuged on glycerol gradients. There was no, or very little, loss of estradiol receptors in the experimental as compared to the control cytosols indicating that AFP is not a constituent of the 8 S estradiol receptor. |
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Authors:
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B Attardi; E Ruoslahti |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Steroids Volume: 30 ISSN: 0039-128X ISO Abbreviation: Steroids Publication Date: 1977 Nov |
Date Detail:
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Created Date: 1978-06-12 Completed Date: 1978-06-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404536 Medline TA: Steroids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 711-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology, metabolism* Brain Chemistry Centrifugation, Density Gradient Cytosol / analysis, metabolism Estradiol / metabolism* Female Immunosorbent Techniques Mice Receptors, Estrogen / analysis*, metabolism Uterus / analysis, cytology, metabolism* alpha-Fetoproteins / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Estrogen; 0/alpha-Fetoproteins; 50-28-2/Estradiol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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