Document Detail


Alpha v beta 6 integrin down-regulates the MMP-13 expression in oral squamous cell carcinoma cells.
MedLine Citation:
PMID:  16024014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The integrin alphavbeta6, a receptor for fibronectin, vitronectin, tenascin and TGF-beta latency-associated peptide (LAP), is not detectable on normal oral epithelium but is neo-expressed in oral squamous cell carcinomas (OSCC) and epithelial dysplasia. Previously it has been shown that alphavbeta6 integrin can up-regulate MMP-3 and -9 expression in OSCC cells. Using beta6-transfected and control OSCC cells we demonstrate that alphavbeta6 integrin down-regulates MMP-13 expression at both mRNA and protein level. Although expressing less MMP-13, beta6-transfected cells were found to have similar collagenolytic activity as control cells and invade at similar levels through type I collagen. Growth of the tumour cells in organotypic culture and confocal microscopy confirmed low levels of MMP-13 in cells with high alphavbeta6 expression. Furthermore, human squamous cell carcinomas of the tongue with high expression of alphavbeta6 showed lower MMP-13 levels than carcinomas with low levels of alphavbeta6. Our results suggest that alphavbeta6 down-regulates MMP-13 expression in OSCC cells and that MMP-13 is not essential for the degradation of type I collagen by OSCC cells.
Authors:
M Ylipalosaari; G J Thomas; M Nystrom; S Salhimi; J F Marshall; V Huotari; T Tervahartiala; T Sorsa; T Salo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  309     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-19     Completed Date:  2005-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-83     Citation Subset:  IM    
Affiliation:
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu and Oulu University Hospital, PO Box 5281, FIN-90014 Oulu, Finland.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / physiology*
Carcinoma, Squamous Cell / enzymology*
Cell Line, Tumor
Collagen Type I / antagonists & inhibitors,  metabolism
Collagenases / antagonists & inhibitors*,  biosynthesis*,  genetics
Down-Regulation* / physiology
Gene Expression Regulation, Neoplastic / physiology*
Humans
Integrins / physiology*
Matrix Metalloproteinase 1 / antagonists & inhibitors,  biosynthesis,  genetics
Matrix Metalloproteinase 13
Matrix Metalloproteinase 8 / antagonists & inhibitors,  biosynthesis,  genetics
Membrane Proteins / antagonists & inhibitors,  biosynthesis,  genetics
Mouth Neoplasms / enzymology*,  metabolism
RNA, Messenger / antagonists & inhibitors,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Collagen Type I; 0/Integrins; 0/Membrane Proteins; 0/RNA, Messenger; 0/integrin alphavbeta6; EC 3.4.24.-/Collagenases; EC 3.4.24.-/MMP13 protein, human; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.34/Matrix Metalloproteinase 8; EC 3.4.24.7/Matrix Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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