| Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. | |
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MedLine Citation:
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PMID: 21255620 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The neurodegenerative process in Parkinson's disease (PD) is accompanied by the presence of a neuroinflammatory response, which has been suggested as one of the principal components involved in PD progression. In this report we assessed the inflammatory potential of alpha-synuclein, a protein central to PD pathogenesis, released by neurons on the mouse microglia cell line BV-2. BV-2 cells were treated with conditioned medium isolated from normal SH-SY5Y cells and clones that over-express WT or mutant A53T alpha-synuclein. Conditioned medium isolated from over-expressing clones induced the transcription and release of pro-inflammatory cytokines. Treatment of SH-SY5Y alpha-synuclein over-expressing cells with MPP+, the active metabolite of the neurotoxin MPTP, increased the inflammatory response in BV-2 cells. In contrast, the direct exposure of BV-2 cells to MPP+ failed to induce an inflammatory response. These results support the hypothesis that WT and A53T alpha-synuclein has an important role in the initiation and maintenance of inflammation in PD, through the activation of a pro-inflammatory response in microglial cells. |
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Authors:
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Lydia Alvarez-Erviti; Yvonne Couch; Jill Richardson; J Mark Cooper; Matthew J A Wood |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-19 |
Journal Detail:
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Title: Neuroscience research Volume: 69 ISSN: 1872-8111 ISO Abbreviation: Neurosci. Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-02-28 Completed Date: 2011-06-15 Revised Date: 2012-01-13 |
Medline Journal Info:
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Nlm Unique ID: 8500749 Medline TA: Neurosci Res Country: Ireland |
Other Details:
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Languages: eng Pagination: 337-42 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. |
Affiliation:
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Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK. l.alvarez@medsch.ucl.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Line Culture Media, Conditioned / metabolism Cytokines / biosynthesis Gene Expression Humans Inflammation / immunology Mice Microglia / immunology*, metabolism Neurons / secretion* Reverse Transcriptase Polymerase Chain Reaction alpha-Synuclein / immunology*, secretion |
| Grant Support | |
ID/Acronym/Agency:
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G-0904//Parkinson's UK; J-0901//Parkinson's UK |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Cytokines; 0/alpha-Synuclein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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