Document Detail

Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line.
MedLine Citation:
PMID:  21255620     Owner:  NLM     Status:  MEDLINE    
The neurodegenerative process in Parkinson's disease (PD) is accompanied by the presence of a neuroinflammatory response, which has been suggested as one of the principal components involved in PD progression. In this report we assessed the inflammatory potential of alpha-synuclein, a protein central to PD pathogenesis, released by neurons on the mouse microglia cell line BV-2. BV-2 cells were treated with conditioned medium isolated from normal SH-SY5Y cells and clones that over-express WT or mutant A53T alpha-synuclein. Conditioned medium isolated from over-expressing clones induced the transcription and release of pro-inflammatory cytokines. Treatment of SH-SY5Y alpha-synuclein over-expressing cells with MPP+, the active metabolite of the neurotoxin MPTP, increased the inflammatory response in BV-2 cells. In contrast, the direct exposure of BV-2 cells to MPP+ failed to induce an inflammatory response. These results support the hypothesis that WT and A53T alpha-synuclein has an important role in the initiation and maintenance of inflammation in PD, through the activation of a pro-inflammatory response in microglial cells.
Lydia Alvarez-Erviti; Yvonne Couch; Jill Richardson; J Mark Cooper; Matthew J A Wood
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-19
Journal Detail:
Title:  Neuroscience research     Volume:  69     ISSN:  1872-8111     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-02-28     Completed Date:  2011-06-15     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  337-42     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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MeSH Terms
Blotting, Western
Cell Line
Culture Media, Conditioned / metabolism
Cytokines / biosynthesis
Gene Expression
Inflammation / immunology
Microglia / immunology*,  metabolism
Neurons / secretion*
Reverse Transcriptase Polymerase Chain Reaction
alpha-Synuclein / immunology*,  secretion
Grant Support
G-0904//Parkinson's UK; G-0910//Parkinson's UK; J-0901//Parkinson's UK
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Cytokines; 0/alpha-Synuclein

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