Document Detail


Alpha-synuclein overexpression reduces gap junctional intercellular communication in dopaminergic neuroblastoma cells.
MedLine Citation:
PMID:  17337120     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alpha-synuclein has been implicated in the pathology of certain neurodegenerative diseases, including Parkinson disease (PD) and dementia with Lewy bodies (LBs). Overexpression of human alpha-synuclein in neuronal cells reduces cell viability, but the precise cellular and molecular mechanisms remain poorly understood. Gap junctional intercellular communication (GJIC) is thought to be essential for maintaining cellular homeostasis and growth control. In the present study, the effect of alpha-synuclein overexpression on GJIC in human dopaminergic neuroblastoma SH-SY5Y cells was investigated. Cells overexpressing wild-type alpha-synuclein were more vulnerable to hydrogen peroxide and 6-hydroxydopamine. GJIC was decreased in cells overexpressing alpha-synuclein. In addition, alpha-synuclein binds directly to connexin-32 (Cx32). As such, the post-translational modification of Cx32 was enhanced in cells overexpressing alpha-synuclein. These findings suggest that alpha-synuclein can modulate GJIC in a dopaminergic neuronal cell line through specific binding to Cx32.
Authors:
Jee Young Sung; Hyun Jung Lee; Eun Il Jeong; Yohan Oh; Joongkyu Park; Kyung-Sun Kang; Kwang Chul Chung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-11
Journal Detail:
Title:  Neuroscience letters     Volume:  416     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  289-93     Citation Subset:  IM    
Affiliation:
Department of Medical Science, Yonsei University College of Medicine, Seoul 120-752, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic Agents / pharmacology
Cell Communication / drug effects,  physiology*
Cell Line, Tumor
Cell Survival / drug effects
Connexins / metabolism
Dopamine / metabolism*
Gap Junctions / metabolism*
Humans
Hydrogen Peroxide / pharmacology
Immunoprecipitation / methods
Isoquinolines / diagnostic use
Mutation / physiology
Neuroblastoma / metabolism*,  pathology
Oxidopamine / pharmacology
Transfection / methods
alpha-Synuclein / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Adrenergic Agents; 0/Connexins; 0/Isoquinolines; 0/alpha-Synuclein; 0/connexin 32; 1199-18-4/Oxidopamine; 7722-84-1/Hydrogen Peroxide; 77944-88-8/lucifer yellow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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