| Alpha integrin subunits regulate human (Caco-2) intestinal epithelial proliferation and phenotype. | |
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MedLine Citation:
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PMID: 10844395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: Cell-matrix interactions influence intestinal epithelial biology, but the whether specific integrin heterodimers exert different effects is unclear. METHODS: We used functional antibodies to investigate effects of the alpha2, alpha3, alpha5, and alpha6 integrin subunits on proliferation and differentiation of human intestinal Caco-2 cells on laminin. Cells seeded onto laminin-coated inserts in defibronectinized medium were treated with functional antibodies or normal IgG for 72 hrs and proliferation, alkaline phosphatase and dipeptidyl dipeptidase specific activity were measured. RESULTS: Caco-2 adhesion to antibody to each alpha-integrin subunit stimulated tyrosine phosphorylation of Focal Adhesion Kinase and paxillin, suggesting that these antibodies intiate integrin-related tyrosine signalling. Proliferation was inhibited by anti-alpha2 and anti-alpha3, but stimulated by anti-alpha6. Alkaline phosphatase and dipeptidyl dipeptidase specific activity were promoted by alpha2 blockade but decreased after a6 blockade. Proliferative blockade using mitomycin C or hydroxyurea prevented the effects of alpha2 ligation on differentiation, but the decrease in alkaline phosphatase specific activity observed after a6 integrin subunit blockade was preserved even after proliferative blockade. CONCLUSION: lntegrin heterodimers modulate human Caco-2 intestinal epithelial biology on laminin in an alpha-subunit specific manner. The different effects of anti-alpha2 and anti-alpha6 may reflect competitition by these antibodies with integrin subunit interactions with laminin as well as initiation of their own signals or different functions for the alpha6 integrin subunit |
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Authors:
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M D Basson; N J Emenaker; M A Sanders |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 10 ISSN: 1015-8987 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2000 |
Date Detail:
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Created Date: 2000-07-20 Completed Date: 2000-07-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 27-36 Citation Subset: IM |
Affiliation:
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Depts. of Surgery, Yale University School of Medicine and CT VA Health Care System, New Haven and West Haven, CT 06520, USA. marc.basson@yale.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies
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immunology Caco-2 Cells Cell Division / physiology* Epithelial Cells / cytology Humans Integrins / immunology, physiology* Intestines / cytology* Phenotype Signal Transduction / physiology |
| Grant Support | |
ID/Acronym/Agency:
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R29DK47051/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Integrins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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