Document Detail

Alpha-defensin overexpression in patients with Bell's palsy and Ramsay Hunt syndrome.
MedLine Citation:
PMID:  22737966     Owner:  NLM     Status:  In-Process    
OBJECTIVES: To investigate the biological factors related to the onset of Bell's palsy, we sought to identify differentially expressed genes in peripheral blood mononuclear cells (PBMCs) and plasma of patients with Bell's palsy and Ramsay Hunt syndrome (RHS).
METHODS: We carried out DNA microarray analyses using PBMCs taken from patients with Bell's palsy at their initial visit and 2 to 4 weeks later. To validate these analyses, we measured the relative messenger RNA levels of alpha-defensin in paired PBMCs by reverse transcription-polymerase chain reaction. The plasma concentrations of alpha-defensin in patients and healthy volunteers were quantified by enzyme-linked immunosorbent assay.
RESULTS: The DNA microarray analysis identified alpha-defensin as a candidate gene related to the onset of Bell's palsy. Reverse transcription-polymerase chain reaction analysis showed that the relative alpha-defensin messenger RNA levels in PBMCs from the later visit were increased at least twofold in 9 of 13 patients (69%) with Bell's palsy and in 4 of 6 patients (67%) with RHS. The plasma alpha-defensin concentrations in the patients with RHS were significantly higher than those in healthy volunteers (p = 0.0013) and in the patients with Bell's palsy (p = 0.0306). Elevations of plasma alpha-defensin were observed in 5 of the 9 patients with Bell's palsy who demonstrated alpha-defensin overexpression in PBMCs.
CONCLUSIONS: alpha-Defensin may be one of the biological factors related to the onset of Bell's palsy and RHS.
Yasushi Furuta; Nobuhiko Oridate; Norihito Takeichi; Satoshi Fukuda; Hirofumi Sawa
Related Documents :
18175786 - The neurotoxicity and safety of treatment with cefepime in patients with renal failure.
7446366 - Arrhythmogenic epilepsy: an hypothesis.
14705836 - Emergence of imported ciguatera in europe: report of 18 cases at the poison control cen...
17290096 - Long-term outcome of guillain-barré syndrome.
1735356 - Splenic and portal venous obstruction in chronic pancreatitis. a prospective longitudin...
603216 - Coagulation disorders in severely and critically injured patients.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of otology, rhinology, and laryngology     Volume:  121     ISSN:  0003-4894     ISO Abbreviation:  Ann. Otol. Rhinol. Laryngol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0407300     Medline TA:  Ann Otol Rhinol Laryngol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  419-25     Citation Subset:  AIM; IM    
Department of Otolaryngology-Head and Neck Surgery, Hokkaido University Graduate School of Medicine, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evaluation of card-type odor identification test for Japanese patients with olfactory disturbance.
Next Document:  Experimental assessment of the importance of amino acid positions identified by an entropy-based cor...