Document Detail


Alpha-beta chimeric oligo-DNA bearing intercalator-conjugated nucleobase inside the linker sequence remarkably improves thermal stability of an alternate-stranded triple helix.
MedLine Citation:
PMID:  15501034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Novel alpha-beta chimeric oligodeoxynucleotides bearing an intercalator-conjugated nucleobase located at the internal 4-nt linker region were synthesized, and their triplex-stabilizing property was examined. The triple helical DNA formed between the modified chimera DNA and double-stranded DNA exhibited remarkable thermal stability; however, the position of the intercalator-conjugated nucleobase had little influence on the stability. Among the examined, modified chimera DNA bearing the two intercalator-conjugated nucleobases at adjacent positions exhibited the highest stability.
Authors:
A T M Zafrul Azam; Minoru Hasegawa; Tomohisa Moriguchi; Kazuo Shinozuka
Related Documents :
14529284 - Structural competition involving g-quadruplex dna and its complement.
7890724 - Determination of 5' and 3' dna triplex interference boundaries reveals the core dna bin...
8932374 - Detection of covalent triplex within human cells.
10625924 - Porphyrins can catalyze the interconversion of dna quadruplex structural types.
12695544 - Probing the role of linker substituents in bisdioxopiperazine analogs for activity agai...
8593684 - Dna polymerase delta of physarum polycephalum.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  14     ISSN:  0960-894X     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-10-25     Completed Date:  2005-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  5747-50     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chimera / genetics
DNA / chemistry,  genetics
Intercalating Agents / chemical synthesis*
Oligodeoxyribonucleotides / chemical synthesis*,  genetics*
Chemical
Reg. No./Substance:
0/Intercalating Agents; 0/Oligodeoxyribonucleotides; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Site-specific PEGylation of proteins containing unnatural amino acids.
Next Document:  Synthesis and cytotoxic activity of substituted luotonin A derivatives.