Document Detail


Alpha-2 adrenoceptor mediating the facilitatory effect of norepinephrine on the glycine response in the spinal dorsal horn neuron of the rat.
MedLine Citation:
PMID:  12798415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effects of norepinephrine (NE) on the glycine-mediated inhibitory response were investigated in neurons acutely dissociated from the rat spinal dorsal horn, using nystatin perforated patch recording mode under voltage-clamp conditions. NE reversibly and concentration dependently facilitated Cl(-) current induced by 3 x 10(-5) M glycine. NE neither changed the reversal potential of the glycine response nor affected the affinity of glycine to its receptor. This effect could be mimicked by clonidine (10(-7) M) and blocked by yohimbine (10(-6) M), respectively. N-[2(methylamino)ethyl]-5-isoquinoline sulfonamide dihydrochloride (H-89), an inhibitor of protein kinase A, effectively mimicked the effect of NE on glycine response, whereas chelerythrine (an inhibitor of protein kinase C) failed. NE further enhanced glycine response even in the presence of chelerythrine or stearoylcarnitine chloride (another inhibitor of protein kinase C) or chelerythrine together with stearoylcarnitine chloride. The present results suggest that alpha2-adrenoceptor is involved in the potentiation of NE on glycine response in freshly isolated spinal dorsal horn neurons. Activation of alpha2-adrenoceptor down-regulates the activity of protein kinase A that results in the potentiation of the glycinergic inhibitory effects within the spinal dorsal horn.
Authors:
Bing Lang; Hui Li; Jie-Fang Kang; Yun-Qing Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  73     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-11     Completed Date:  2003-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  893-905     Citation Subset:  IM    
Affiliation:
Department of Anatomy, The Fourth Military Medical University, Xi'an, 710032, PR China.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids
Animals
Benzophenanthridines
Clonidine / pharmacology
Dose-Response Relationship, Drug
Drug Combinations
Electric Conductivity
Enzyme Inhibitors / pharmacology
Glycine / pharmacology*
Isoquinolines / pharmacology
Neurons / cytology,  drug effects*,  metabolism
Norepinephrine / pharmacology*
Nystatin / pharmacology
Patch-Clamp Techniques
Phenanthridines / pharmacology
Posterior Horn Cells / cytology,  drug effects*,  metabolism
Rats
Rats, Sprague-Dawley
Receptors, Adrenergic, alpha-2* / metabolism
Sulfonamides*
Yohimbine / pharmacology
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Benzophenanthridines; 0/Drug Combinations; 0/Enzyme Inhibitors; 0/Isoquinolines; 0/Phenanthridines; 0/Receptors, Adrenergic, alpha-2; 0/Sulfonamides; 127243-85-0/H 89; 1400-61-9/Nystatin; 146-48-5/Yohimbine; 34316-15-9/chelerythrine; 4205-90-7/Clonidine; 51-41-2/Norepinephrine; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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