| Alpha-1D adrenoceptors are involved in reserpine-induced supersensitivity of rat tail artery. | |
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MedLine Citation:
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PMID: 15159276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. We examined reserpine-induced chemical denervation supersensitivity with special reference to alpha-1 adrenoceptor (AR) subtypes. 2. Chronic treatment with reserpine for 2 weeks depleted noradrenaline in the tail artery and spleen of rats. Noradrenaline in the thoracic aorta was negligible before and after reserpine treatment. 3. The treatment with reserpine produced supersensitivity in the contractile responses of the rat tail artery to phenylephrine, 5-HT and KCl, resulting in leftward shift of concentration-response curves (11.6-, 2.5- and 1.1-fold at EC(50) value, respectively). These results suggest a predominant sensitization of the alpha-1 AR-mediated response by reserpine treatment. 4. BMY 7378 at a concentration (30 nm) specific for blocking the alpha-1D AR subtype, but not KMD-3213 at a concentration (10 nm) selective for blocking the alpha-1A AR subtype, inhibited the supersensitivity of the phenylephrine-induced response in the reserpine-treated artery. On the other hand, the response to phenylephrine in reserpine-untreated artery was selectively inhibited by the same concentration of KMD-3213, but not by BMY 7378. Prazosin, a subtype-nonselective antagonist, blocked the responses to phenylephrine with the same potency, regardless of reserpine treatment. 5. In the thoracic aorta and spleen, no supersensitivity was produced in the responses to phenylephrine by reserpine treatment. 6. In a tissue segment-binding study using [(3)H]-prazosin, the total density and affinity of alpha-1 ARs in the rat tail artery were not changed by treatment with reserpine. However, alpha-1D AR with high affinity for BMY 7378 was significantly detected in reserpine-treated tail artery, in contrast to untreated artery. Decreases in alpha-1A AR with high affinity for KMD-3213 and alpha-1B AR with low affinities for KMD-3213 and BMY 7378 were also estimated in reserpine-treated tail artery. 7. Alpha-1D AR mRNA in rat tail artery increased to three-folds by reserpine treatment, whereas the levels of alpha-1A and 1B mRNAs were not significantly changed. 8. The present results suggest that chronic treatment with reserpine affects the expression of alpha-1 AR subtypes of rat tail artery and that the induction of alpha-1D ARs with high affinity for catecholamines is in part associated with reserpine-induced supersensitivity. |
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Authors:
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Naoyuki Taki; Takashi Tanaka; Li Zhang; Fumiko Suzuki; Malika Israilova; Takanobu Taniguchi; Yasuko Hiraizumi-Hiraoka; Kazumasa Shinozuka; Masaru Kunitomo; Ikunobu Muramatsu |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2004-05-24 |
Journal Detail:
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Title: British journal of pharmacology Volume: 142 ISSN: 0007-1188 ISO Abbreviation: Br. J. Pharmacol. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-18 Completed Date: 2005-01-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7502536 Medline TA: Br J Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 647-56 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Medicine, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Thoracic / chemistry, drug effects Arteries / drug effects, metabolism Dose-Response Relationship, Drug Drug Administration Schedule Drug Hypersensitivity* Gene Expression / drug effects, genetics Indoles / administration & dosage Japan Male Phenylephrine / pharmacology Piperazines / pharmacology Potassium Chloride / pharmacology Prazosin / pharmacology RNA, Messenger Rats Rats, Wistar Receptors, Adrenergic, alpha-1 / drug effects, physiology* Reserpine / pharmacology* Serotonin / pharmacology Spleen / chemistry, drug effects Tail / blood supply*, drug effects, metabolism Time Factors Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 0/KMD 3213; 0/Piperazines; 0/RNA, Messenger; 0/Receptors, Adrenergic, alpha-1; 0/adrenergic receptor alpha(1d); 10028-17-8/Tritium; 19216-56-9/Prazosin; 21102-94-3/BMY 7378; 50-55-5/Reserpine; 50-67-9/Serotonin; 59-42-7/Phenylephrine; 7447-40-7/Potassium Chloride |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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