| Alpha-1-Antitrypsin Acts as a Preeclampsia-Related Protein: A Proteomic Study. | |
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MedLine Citation:
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PMID: 22414876 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aims: To screen the preeclampsia-related protein by proteomics. Methods: Proteomics was performed to identify differential protein expression profiles between normal full-term pregnancy, early-onset severe preeclampsia (ES-PE) or late-onset severe preeclampsia (LS-PE; n = 10 per group). Real-time quantitative PCR and immunohistochemistry were conducted to confirm the expression of α(1)-antitrypsin (α(1)-AT) in the decidual tissues of different subjects. ELISA was employed to detect the α(1)-AT content in the peripheral blood of 90 women (n = 30 per group). Results: We successfully constructed two-dimensional electrophoresis maps of decidual tissues, and a total of 20 differentially expressed proteins were identified. The α(1)-AT expression was different among the three groups. The normal full-term pregnancy women expressed the most α(1)-AT, and the LS-PE women expressed the least amount of α(1)-AT. The difference in the α(1)-AT expression was consistent with the proteomics data. The peripheral α(1)-AT content was the highest in the normal full-term pregnancy group (1.85 ± 0.15 g/l), moderate in the ES-PE group (0.77 ± 0.14 g/l) and lowest in the LS-PE group (0.42 ± 0.07 g/l; p < 0.05). Conclusion: Using 2D PAGE, we identified twenty proteins with significantly altered expression in PE. These differentially expressed proteins include prevention protein, in which α(1)-AT is downregulated in PE. |
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Authors:
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Ya-Ling Feng; Chang-Ju Zhou; Xiao-Mao Li; Xiao-Qin Liang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-08 |
Journal Detail:
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Title: Gynecologic and obstetric investigation Volume: - ISSN: 1423-002X ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7900587 Medline TA: Gynecol Obstet Invest Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 S. Karger AG, Basel. |
Affiliation:
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Department of Obstetrics and Gynecology, Shenzhen Baoan People's Hospital, Shenzhen, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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