Document Detail


Alosetron, cilansetron and tegaserod modify mesenteric but not colonic blood flow in rats.
MedLine Citation:
PMID:  19785647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: As the use of the 5-HT(3) receptor antagonist alosetron (GlaxoSmithKline) and the 5-HT(4) receptor agonist tegaserod (Novartis) in patients with irritable bowel syndrome has been associated with cases of ischaemic colitis, the effects of alosetron, cilansetron (Solvay) and tegaserod on the rat splanchnic circulation were evaluated.
EXPERIMENTAL APPROACH: Phenobarbital-anaesthetised rats were instrumented to record blood flow in the superior mesenteric artery and transverse colon and to calculate mesenteric and colonic vascular conductance.
KEY RESULTS: Intravenous alosetron (0.03-0.3 mg.kg(-1)) did not alter blood pressure or heart rate but reduced mesenteric blood flow and vascular conductance by 15-20%. This activity profile was also seen after intraduodenal alosetron and shared by the 5-HT(3) receptor antagonist cilansetron. In contrast, blood flow, vascular conductance and intraluminal pressure in the colon were not modified by alosetron and cilansetron. Intravenous or intraduodenal tegaserod (0.3-1.0 mg.kg(-1)) had no inhibitory effect on mesenteric and colonic blood flow. Peroral treatment of rats with alosetron or tegaserod for 7 days did not modify mesenteric haemodynamics at baseline and after blockade of nitric oxide synthesis. Mild inflammation induced by dextran sulphate sodium failed to provoke a vasoconstrictor effect of cilansetron in the colon.
CONCLUSIONS AND IMPLICATIONS: Alosetron and cilansetron, not tegaserod, caused a small and transient constriction of the rat mesenteric vascular bed, whereas blood flow in the colon remained unaltered. The relevance of these findings to the treatment-associated occurrence of ischaemic colitis in patients with irritable bowel syndrome remains open.
Authors:
Evelin Painsipp; Anaid Shahbazian; Peter Holzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-25
Journal Detail:
Title:  British journal of pharmacology     Volume:  158     ISSN:  1476-5381     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-28     Completed Date:  2010-02-12     Revised Date:  2011-04-06    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1210-26     Citation Subset:  IM    
Affiliation:
Research Unit of Translational Neurogastroenterology, Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic alpha-Agonists / pharmacology
Animals
Blood Pressure / drug effects
Carbazoles / administration & dosage,  adverse effects*
Carbolines / administration & dosage,  adverse effects*
Clonidine / pharmacology
Colitis / physiopathology
Colon / blood supply,  drug effects*
Fasting
Female
Heart Rate / drug effects
Indoles / administration & dosage,  adverse effects*
Mesenteric Artery, Superior / drug effects,  physiology
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors
Pyridines / administration & dosage,  adverse effects*
Rats
Rats, Sprague-Dawley
Regional Blood Flow / drug effects
Serotonin 5-HT3 Receptor Antagonists*
Serotonin 5-HT4 Receptor Antagonists*
Splanchnic Circulation / drug effects*
Vasoconstrictor Agents / pharmacology
Vasodilator Agents / pharmacology
Grant Support
ID/Acronym/Agency:
L 25-B05//Austrian Science Fund FWF
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Carbazoles; 0/Carbolines; 0/Indoles; 0/Pyridines; 0/Serotonin 5-HT3 Receptor Antagonists; 0/Serotonin 5-HT4 Receptor Antagonists; 0/Vasoconstrictor Agents; 0/Vasodilator Agents; 120635-74-7/cilansetron; 122852-42-0/alosetron; 145158-71-0/tegaserod; 4205-90-7/Clonidine; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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