Document Detail


Allyl isothiocyanate influences cell adhesion, migration and metalloproteinase gene expression in SK-Hep1 cells.
MedLine Citation:
PMID:  18997101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Allyl isothiocyanate (AITC) has been reported to exhibit antimetastatic activity, but the mechanism remains unclear. The objective of this study was to determine the effect of AITC on cell adhesion, migration, and metalloproteinase gene expression in SK-Hep1 human hepatoma cells. The gene expression profiles of SK-Hep1 cells were obtained by using the HG-U133A Affymetrix GeneChip human genome array containing 14,500 human genes. Twenty antimetastatic genes including COL4A3, ADAMDEC1, CAPN10, CD14, and ITGB1BP3 were over expressed, while the expression of 35 genes such as COL8A1, MYBPC1, ST14, and SOS2 were down-regulated. Semiquantitative RT-PCR confirmed these results in mRNA levels. Based on these in vitro results, it can be concluded that AITC might be potentially useful in suppressing tumor cell migration and MMP expression.
Authors:
Eun-Sun Hwang; Gun Hee Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-07
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  234     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-25     Completed Date:  2009-02-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  105-11     Citation Subset:  IM    
Affiliation:
Center for Agricultural Biomaterials, College of Agriculture and Life Sciences, Seoul National University, Shillim-dong, Gwanak-gu, Korea. ehwang@mifaff.go.kr
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / genetics,  pathology*
Cell Adhesion / drug effects*
Cell Line, Tumor
Cell Movement / drug effects*
DNA Primers
Gene Expression Regulation, Enzymologic / drug effects*
Gene Expression Regulation, Neoplastic / drug effects
Humans
Isothiocyanates / pharmacology*
Liver Neoplasms / genetics,  pathology*
Metalloproteases / drug effects*,  genetics*
Oligonucleotide Array Sequence Analysis
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Isothiocyanates; 57-06-7/allyl isothiocyanate; EC 3.4.-/Metalloproteases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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