Document Detail


Allyl sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis.
MedLine Citation:
PMID:  20459099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), extracted from crushed garlic by steam-distillation, have been reported to provide the anticancer activity in several cancer types. However, their mechanisms of effects on skin cancer cells remain unclear. Therefore, we used human melanoma A375 cells and basal cell carcinoma cells as the models to elucidate the effects of these three allyl sulfides. Basal cell carcinoma (BCC) is known to be the most prevalent type of skin cancer, and melanoma is the most lethal form. We found that DATS revealed better growth inhibition of A375 and BCC cells than DADS and DAS did. We further demonstrated that DATS increased intracellular reactive oxygen species (ROS) generation, induced cytosolic Ca(2+) mobilization, and decreased mitochondrial membrane potential (DeltaPsim). Western blot results showed the concordance for the expression of molecules involved in G(2)/M arrest and apoptosis observed by cell cycle and cell viability analysis. Moreover, we detected the activation of p53 pathway in response to the oxidative DNA damage. DATS also displayed selective target of growth inhibition between skin cancer cells and normal keratinocyte HaCaT cells. Taken together, these results suggest that DATS is a potential anticancer compound for skin cancer.
Authors:
Hsiao Chi Wang; Jen-Hung Yang; Shu-Chen Hsieh; Lee-Yan Sheen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-02     Completed Date:  2010-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7096-103     Citation Subset:  IM    
Affiliation:
Graduate Institute of Food Science and Technology, National Taiwan University, No 1, Sec 4, Roosevelt Road, Taipei 106, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Allyl Compounds / pharmacology*
Apoptosis / drug effects*
Cell Cycle / drug effects*
Cell Division / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
DNA Damage / drug effects*
Down-Regulation / drug effects*
G2 Phase / drug effects
Humans
Reactive Oxygen Species / metabolism
Skin Neoplasms / drug therapy,  genetics,  metabolism,  physiopathology*
Sulfides / pharmacology*
Tumor Suppressor Protein p53 / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Allyl Compounds; 0/Reactive Oxygen Species; 0/Sulfides; 0/Tumor Suppressor Protein p53; 592-88-1/allyl sulfide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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