Document Detail


Allografts of CNS tissue possess a blood-brain barrier. II. Angiogenesis in solid tissue and cell suspension grafts.
MedLine Citation:
PMID:  2013306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Angiogenesis and patency of blood vessels were analyzed qualitatively in solid CNS and peripheral tissue syngeneic, allogeneic, and xenogeneic grafts and in individual cell suspension grafts of astrocytes, fibroblasts, PC12, and three additional tumor cell lines placed intracerebrally in adult host mice. Postgrafting survival times were 1 day through 4 weeks. The patency of graft vessels was determined in sections from immersion-fixed tissues incubated to reveal the endogenous peroxidase activity of host red cells trapped within the lumen of blood vessels. Additionally, horseradish peroxidase (HRP) was administered intravenously to live hosts; HRP labels host brain and graft vessels on the luminal surface and reveals the presence or absence of a blood-brain barrier (BBB) within the grafts. The origins of blood vessels supplying solid tissue xenografts were identified immunohistochemically with primary antibodies against host (athymic AKR mice) and donor (fetal Lewis rats) major histocompatibility complex (MHC) class I. Blood vessels supplying solid CNS grafts at 1-7 days post-transplantation were identified ultrastructurally and possessed interendothelial tight junctional complexes; however, they were not perfused with either host blood or blood-borne HRP prior to 8 days. Graft vessels at 10 days were outlined consistently by peroxidase-positive red cells in immersion-fixed material and labeled with blood-borne HRP. These vessels provided a BBB to the circulating HRP and exhibited interendothelial tight junctions. Evidence of angiogenesis within solid anterior pituitary grafts and the variety of cell suspension grafts was obtained prior to 3 days post-transplantation in immersion-fixed preparations; the vessels, with the notable exception of those supplying astrocyte cell suspensions, failed to present a BBB to blood-borne peroxidase. Endothelia in the solid pituitary allografts and the PC12 cell grafts were highly fenestrated and exhibited open interendothelial junctions; those in the tumor and fibroblast cell grafts, for the most part, appeared nonfenestrated, and many possessed open interendothelial junctional complexes. Immunostaining for host and donor MHC class I revealed that donor blood vessels predominate over host vessels in CNS xenografts and supply pituitary xenografts exclusively; in both preparations, donor vessels were not identified within the host CNS. Because cell suspension grafts were derived from endothelia-free preparations grown in culture, blood vessels supplying these grafts were necessarily of host CNS origin and manifested a morphological transformation from a BBB to a non-BBB endothelium. The data suggest that angiogenesis in solid CNS grafts placed into the adult host CNS, compared to similarly placed solid peripheral tissue/cell suspension grafts, is not rapid.(ABSTRACT TRUNCATED AT 400 WORDS)
Authors:
R D Broadwell; H M Charlton; P S Ebert; W F Hickey; Y Shirazi; J Villegas; A L Wolf
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental neurology     Volume:  112     ISSN:  0014-4886     ISO Abbreviation:  Exp. Neurol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-05-14     Completed Date:  1991-05-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-28     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Maryland School of Medicine, Baltimore 21201.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Blood-Brain Barrier*
Brain Tissue Transplantation / physiology*
Cerebrovascular Circulation*
Endothelium, Vascular / physiology,  ultrastructure
Fetal Tissue Transplantation / physiology
Intercellular Junctions / ultrastructure
Mice
Mice, Inbred AKR
Mice, Nude
Parietal Lobe / surgery*
Pituitary Gland, Anterior / surgery*
Preoptic Area / surgery*
Rats
Rats, Inbred Lew
Transplantation, Heterologous
Transplantation, Homologous
Transplantation, Isogeneic
Grant Support
ID/Acronym/Agency:
FOSTW04285/ST/OHS HRSA HHS; NS18030/NS/NINDS NIH HHS; NS27321/NS/NINDS NIH HHS

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