Document Detail


Allogeneic dendritic cell induction of HIV-specific cytotoxic T lymphocyte responses from T cells of HIV type 1-infected and uninfected individuals.
MedLine Citation:
PMID:  8989425     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The potential benefit of T cell-based vaccination for HIV-1 infection remains to be determined. Cytotoxic T lymphocytes (CTLs) appear to clear substantial populations of HIV-1 virus in vivo, although CTL activity may contribute to the decline in CD4+ T cell count observed in the course of the disease. To investigate further the role of specific CTL responses in the control of HIV-1 replication, we raised primary CTL lines against a panel of conserved HIV-1 epitopes using blood-derived dendritic cells as antigen-presenting cells (APCs). Specific primary human CTL responses were induced against HLA-A*0201-restricted peptides with dendritic cells from HIV-1-seronegative donors. This method of immunization elicited cytotoxic activities capable of recognizing endogenously processed antigen. The CTL induction protocol was extended in order to explore the capacity of HLA-matched allogeneic dendritic cells to evoke novel CTL responses in T cells from an HIV-seropositive asymptomatic individual. Allogeneic peptide-pulsed dendritic cells from a healthy sibling were capable of eliciting a CTL response directed against an HIV epitope (env814: SLLNATDIAV) that was initially not detected in the CTL effector population of the HIV-1-infected patient. The possibility of manipulating CTL specificity directed against multiple conserved HIV-1 epitopes represents a significant step in the evaluation of T cell-based vaccination for treatment of disease.
Authors:
M Dupuis; M V Peshwa; C Benike; S K Kundu; E G Engleman; W C Van Schooten; T C Merigan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  13     ISSN:  0889-2229     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-19     Completed Date:  1997-03-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  33-9     Citation Subset:  IM; X    
Affiliation:
Center for AIDS Research, Stanford University Medical Center, California 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Viral / immunology
Cell Line
Dendritic Cells / immunology*
HIV Seropositivity / immunology*
HIV-1 / immunology*
HLA-A Antigens / immunology
Humans
Nuclear Family
Oligopeptides / immunology
T-Lymphocytes / immunology
T-Lymphocytes, Cytotoxic / immunology*
Grant Support
ID/Acronym/Agency:
AI 36608/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Viral; 0/HLA-A Antigens; 0/Oligopeptides

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