Document Detail


Allogeneic bone marrow transplantation using stem cells fractionated by lectins: VI, in vitro analysis of human and monkey bone marrow cells fractionated by sheep red blood cells and soybean agglutinin.
MedLine Citation:
PMID:  6109148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A procedure was developed for the isolation from human bone marrow of a cell fraction enriched for haematopoietic precursors and depleted of T lymphocytes. T cells are eliminated from bone marrow by rosetting with sheep red blood cells, followed by differential agglutination of residual T lymphocytes in the non-rosetting population by the lectin, soybean agglutinin. The fraction unagglutinated by the lectin contains a high proportion of colony-forming cells and non detectable T cell alloreactivity in vitro. Similar results were obtained with monkey bone-marrow cells, suggesting that monkeys can be used for evaluation of this fractionation technique for bone-marrow transplantation across histocompatibility barriers.
Authors:
Y Reisner; N Kapoor; R J O'Reilly; R A Good
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lancet     Volume:  2     ISSN:  0140-6736     ISO Abbreviation:  Lancet     Publication Date:    1980 Dec 20-27
Date Detail:
Created Date:  1981-03-17     Completed Date:  1981-03-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985213R     Medline TA:  Lancet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1320-4     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Erythrocytes
Haplorhini / immunology
Hematopoietic Stem Cell Transplantation*
Humans
Lymphocyte Activation
Lymphocyte Depletion
Phytohemagglutinins
Plant Lectins
Rosette Formation
Soybeans
Transplantation, Homologous
Grant Support
ID/Acronym/Agency:
CA-08748/CA/NCI NIH HHS; CA-17404/CA/NCI NIH HHS; CA-23766/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Phytohemagglutinins; 0/Plant Lectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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