Document Detail


Allogeneic bone marrow cocultured with human islets significantly improves islet survival and function in vivo.
MedLine Citation:
PMID:  23416682     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A significant barrier to islet transplantation is the rapid loss of human islet function in vivo. The present study evaluates whether bone marrow (BM) could be used to support human islet survival and function in vivo.
METHODS: We cocultured human islets and BM for 3 weeks before transplantation into the left subrenal capsule of diabetic severe combined immunodeficient mice.
RESULTS: The cocultured human islets before transplantation demonstrated improved viability, increased size, and migration capacity in vitro. After 4 months, animals transplanted with precultured BM/islets exhibited euglycemia and detectable human insulin levels (157 μU/mL), whereas no human insulin was detected in the islet-only transplantation group. Furthermore, the removal of the transplants on day 126 resulted in hyperglycemia, indicating that the reduction of blood glucose was dependent on the transplants. Diabetic mice transplanted with BM/islets demonstrated the longest survival period (130 vs. 40 days for those with islet-only transplants). The transplanted BM/islets showed signs of vascularization and migration from the renal capsule into medulla.
CONCLUSIONS: Our results suggest that BM precultured with human islets may enhance the survival and function of transplanted islets, thus significantly improving the therapeutic efficacy of islet transplantation for type 1 diabetes.
Authors:
John Z Q Luo; Fang Xiong; A Samer Al-Homsi; Camillo Ricordi; Luguang Luo
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  95     ISSN:  1534-6080     ISO Abbreviation:  Transplantation     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-05-13     Revised Date:  2014-04-25    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  801-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / metabolism
Bone Marrow Cells / cytology*
Cell Survival
Cells, Cultured
Coculture Techniques / methods*
Diabetes Mellitus, Experimental / metabolism,  therapy*
Diabetes Mellitus, Type 1 / therapy
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay / methods
Fluorescent Dyes / pharmacology
Humans
Hyperglycemia / metabolism
Islets of Langerhans / cytology*
Islets of Langerhans Transplantation / methods*
Male
Mice
Mice, Inbred BALB C
Mice, SCID
Time Factors
Transplantation, Homologous
Grant Support
ID/Acronym/Agency:
P20 RR018757/RR/NCRR NIH HHS; P20RR018757/RR/NCRR NIH HHS; R01 DK097380/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Fluorescent Dyes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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