| Allergenicity study of EGFP-transgenic chicken meat by serological and 2D-DIGE analysis. | |
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MedLine Citation:
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PMID: 20188780 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genetically modified (GM) foods must be tested for safety, including by allergenicity tests to ensure that they do not contain new allergens or higher concentrations of known allergens than the same non-GM foods. In this study experimentally developed EGFP-transgenic chickens were used and evaluated the allergenicity of meat from the chicken based on a serological and two-dimensional difference gel electrophoresis (2D-DIGE) analysis. For the serological analysis, a Western blotting with allergen-specific antibodies and a proteomic analysis of chicken meat allergens with patients' sera, a so-called allergenome analysis, were used. The allergenome analysis allowed us to identify five IgE-binding proteins in chicken meat, including a known allergen, chicken serum albumin, and no qualitative difference in their expressions between the GM and non-GM chicken meat was found. Results of the 2D-DIGE analysis showed that none of the IgE-binding proteins in chicken meat were significantly changed in expression levels between non-GM and GM chicken, and only 3 of the 1500 soluble protein spots including green fluorescence protein were markedly different as a result of gene transfer. These above results showed that the combination of serological and 2D-DIGE analysis is a valid method of evaluating quality and quantity of allergens in GM foods. |
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Authors:
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Rika Nakamura; Ryosuke Nakamura; Mikiharu Nakano; Kenjiro Arisawa; Ryo Ezaki; Hiroyuki Horiuchi; Reiko Teshima |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-25 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 48 ISSN: 1873-6351 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-07-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 1302-10 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Division of Novel Foods and Immunochemistry, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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adverse effects*,
immunology Animals Animals, Genetically Modified / immunology* Chickens Electrophoresis, Gel, Two-Dimensional Food Hypersensitivity / blood, etiology, immunology* Food, Genetically Modified / adverse effects* Gene Expression Profiling Green Fluorescent Proteins / biosynthesis, genetics Humans Immunoglobulin E / analysis, metabolism Meat / adverse effects* Muscle Proteins / immunology Peptide Mapping Proteomics Serum Albumin / analysis, immunology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tissue Extracts / adverse effects*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Muscle Proteins; 0/Serum Albumin; 0/Tissue Extracts; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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