Document Detail


Allergen specificity of skin-infiltrating T cells is not restricted to a type-2 cytokine pattern in chronic skin lesions of atopic dermatitis.
MedLine Citation:
PMID:  8941677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The majority of allergen-specific T cells derived from inhalant allergen patch test lesions in patients with atopic dermatitis were previously found to produce a restricted type-2 cytokine pattern. Recent studies, however, have revealed that in chronic eczematous skin lesions of patients with atopic dermatitis, expression of the type-1 cytokine interferon-gamma predominates. To evaluate cytokine production by allergen-specific T cells in chronic atopic dermatitis, we established house dust mite (Dermatophagoides pteronyssinus)-specific T-cell clones from the dermis of chronic skin lesions of sensitized adult patients with atopic dermatitis. Frequencies of skin-derived T cells proliferating in the presence of Dermatophagoides pteronyssinus were between one in 138 and one in 4255, indicating that only a minority of skin-infiltrating T cells are allergen specific. When these cells were analyzed for their capacity to produce interferon-gamma, the majority (71%) of these cells were found to express interferon-gamma mRNA and to secrete interferon-gamma protein, either alone or in combination with interleukin-4. Phenotypic analysis revealed that 15% of skin-infiltrating allergen-specific T cells were CD8+. No selection of Vbeta elements was detected in Dermatophagoides pteronyssinus-specific T-cell clones. These studies demonstrate that allergen specificity of skin-infiltrating T cells is not restricted to a type-2 cytokine pattern in lesional atopic dermatitis. The notion that the majority of allergen-specific, skin-infiltrating T cells are capable of producing interferon-gamma further supports the concept that interferon-gamma expression has major pathogenetic relevance for the chronic phase of atopic dermatitis.
Authors:
T Werfel; A Morita; M Grewe; H Renz; U Wahn; J Krutmann; A Kapp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  107     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-14     Completed Date:  1997-01-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  871-6     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Hannover Medical School, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Allergens / immunology*
Animals
Dermatitis, Atopic / immunology*
Dust
Humans
Immunity, Cellular
Interferon-gamma / metabolism*
Interleukin-4 / metabolism*
Mites / immunology*
Phenotype
Polymerase Chain Reaction / methods
RNA, Messenger / metabolism
T-Lymphocyte Subsets / immunology,  metabolism*
Chemical
Reg. No./Substance:
0/Allergens; 0/Dust; 0/RNA, Messenger; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

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