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All-trans retinoic acid protects hepatocellular carcinoma cells against serum starvation induced cell death by up-regulating collagen 8A2.
MedLine Citation:
PMID:  23298258     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
As a therapeutic or chemo-preventative agent to various cancers, all-trans retinoic acid (atRA) has been reported to inhibit the growth, induce apoptosis or cause differentiation. It was found that atRA could protect hepatocellular carcinoma (HCC) cells against cell death induced by serum starvation in the present study. Furthermore, it was found that atRA could enhance the cell adhesion but no effect on cell cycle and apoptosis. By Illumina Human HT-12 v4 expression microarray, 207 up-regulated and 173 down-regulated genes were identified in HepG2 cells treated by atRA. The most up-regulated genes are cytochrome P450 family 26 subfamily A polypeptide 1(CYP26A1), histidine triad nucleotide binding protein 3 (HINT3), miR-1282 and cytochrome P450 family 26 subfamily B polypeptide 1(CYP26B1), which showed > 5 fold more expression. By Gene Ontology analysis, the most significance was enriched in extracellular matrix (ECM) related molecular functions and cellular component in up-regulated genes. The up-regulation of collagen 8A2 (COL8A2) was further confirmed in the quantitative RT-PCR and Western blotting. Knockdown of COL8A2 blocked the enhancement in the early stage of cell adhesion by atRA treatment. Re-expression of COL8A2 in COL8A2 knock-downed HCC cells reversed the effect of siRNA-COL8A2. In addition, COL8A2 could increase HCC cell migration and invasion. Thus, COL8A2 was identified as the key protein involved in the enhancement of cell adhesion of atRA under serum free condition. In the conclusion, atRA protects HCC cells against serum starvation induced cell death by enhancing cell adhesion, and COL8A2 plays an important role in HCC cell migration and invasion. © 2013 The Authors Journal compilation © 2013 FEBS.
Authors:
Wen Wang; Gang Xu; Cui-Ling Ding; Lan-Juan Zhao; Ping Zhao; Hao Ren; Zhong-Tian Qi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  The FEBS journal     Volume:  -     ISSN:  1742-4658     ISO Abbreviation:  FEBS J.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101229646     Medline TA:  FEBS J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors Journal compilation © 2013 FEBS.
Affiliation:
Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, National Innovation Alliance for Hepatitis & Liver Cancer, Second Military Medical University, Shanghai, 200433, China.
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