| All-trans- and 9-cis-retinoic acids activate the human cyclooxynase-2 gene: a role for DR1 as RARE or RXRE. | |
| | |
MedLine Citation:
|
PMID: 20376700 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The human COX-2 promoter contains a direct repeat 1 (DR1) which was shown to confer responsiveness to PPARs. We found that in AN(3)CA and F9 cells, this hCOX-2 DR1 mediates responsiveness to all-trans-retinoic acid (tRA) or 9-cis-retinoic acid (9cRA), but this effect was suppressed by PPARδ. Truncated PPARδ lacking the activation domain AF2 cannot suppress RA-induced activation of the hCOX-2 gene via DR1, suggesting that cofactor recruitment by AF2 is required for the suppression by PPARδ. Gel shift assay showed that PPAR/RXR, RARβ/RXR, and RXR/RXR, bind to hCOX-2 DR1, revealing the promiscuity of this DR1. Particularly, RXR homodimer was able to bind to this DR1 only in the presence of 9cRA. Our results established that tRA and 9cRA are potent inducers of hCOX-2 and that the hCOX-2 DR1 could either serve as RARE or RXRE depending on cellular contexts. |
| | |
Authors:
|
Kyuyong Han; Irene Moon; Hyunjung J Lim |
Publication Detail:
|
Type: Journal Article Date: 2010-04-08 |
Journal Detail:
|
Title: Molecular biology reports Volume: 38 ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-01-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 833-40 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, IBST, RCTC, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossu...
Next Document: Real-time reverse transcription-PCR expression profiling of porcine troponin I family in three diffe...