| All amacrine cells in the rabbit retina possess AMPA-, NMDA-, GABA-, and glycine-activated currents. | |
| | |
MedLine Citation:
|
PMID: 15259569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Physiological properties of ligand-activated currents were characterized for morphologically identified AII amacrine cells in the rabbit retina by using whole-cell recordings in a superfused retina slice preparation. The AII amacrine cells were identified based on their distinct narrow-field, bistratified morphology. In the present study, the whole-cell recordings from AII amacrine cells synaptically isolated from presynaptic influences demonstrated the presence of glutamate AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid) receptors, but no kainate receptors. The presence of only AMPA receptors on rabbit AII amacrine cells is in contrast to an earlier study on rabbit AII amacrine cells by Bloomfield and Xin (2000), but consistent with previous studies on rat AII amacrine cells. In addition, NMDA (N-methyl-D-aspartate) -activated currents blocked by the NMDA antagonist D-AP7 (D-2-amino-7-phosphonoheptanoic acid) were found on the AII amacrine cells. These most likely extrasynaptic NMDA-activated currents were attenuated by the presence of Co2+ interacting with Mg2+ and Ca2+ as they competed for divalent cation-binding sites within the NMDA channel. AII amacrine cells also possessed GABA (gamma-aminobutyric acid) -activated currents that were unaffected by the GABAc receptor antagonist TPMPA (1,2,5,6-tetrahydropyridine-4-yl methylphosphinic), but were completely blocked by the GABA(A) antagonist bicuculline. This indicates that the major inhibitory inputs were mediated by only GABA(A) receptors located directly on the AII amacrine cells. Furthermore, although the AII amacrine cells were glycinergic amacrine cells, they also possessed glycine-activated currents that may be mediated by autoreceptors. |
| | |
Authors:
|
Chengwen Zhou; Ramon F Dacheux |
Related Documents
:
|
17561939 - Delayed rectifier outward k+ current mediates the migration of rat cerebellar granule c... 6329459 - Procaine actions on tetrodotoxin sensitive and insensitive leech neurons. 12825299 - Cytocentering: a novel technique enabling automated cell-by-cell patch clamping with th... 12920599 - Pseudomonas aeruginosa activates cl- channels in host epithelial cells. 10482749 - Loss of ia expression and increased excitability in postnatal rat cajal-retzius cells. 1726559 - Identification of cultured cells expressing ligand-gated cationic channels. 8322459 - Renal cell carcinoma with osteoclast-like giant cells. 7796889 - Cell cycle-related changes in f-actin distribution are correlated with glycolytic activ... 14530519 - Pendrin transporter carries out iodide uptake into mcf-7 human mammary cancer cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Visual neuroscience Volume: 21 ISSN: 0952-5238 ISO Abbreviation: Vis. Neurosci. Publication Date: 2004 Mar-Apr |
Date Detail:
|
Created Date: 2004-07-19 Completed Date: 2004-08-12 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8809466 Medline TA: Vis Neurosci Country: England |
Other Details:
|
Languages: eng Pagination: 181-8 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, University of Alabama at Birmingham, Birmingham 35294-0009, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amacrine Cells
/
drug effects,
metabolism* Animals Cell Membrane / drug effects, metabolism Electrophysiology Excitatory Amino Acid Agonists / pharmacology Excitatory Amino Acid Antagonists / pharmacology Patch-Clamp Techniques Rabbits Receptors, AMPA / metabolism* Receptors, GABA / metabolism* Receptors, Glycine / metabolism* Receptors, N-Methyl-D-Aspartate / metabolism* Retina / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
EY-03039/EY/NEI NIH HHS; EY-07033/EY/NEI NIH HHS; EY03011/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Excitatory Amino Acid Agonists; 0/Excitatory Amino Acid Antagonists; 0/Receptors, AMPA; 0/Receptors, GABA; 0/Receptors, Glycine; 0/Receptors, N-Methyl-D-Aspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of the cervico-ocular reflex in the "flying" pigeon: interactions with the optokineti...
Next Document: Is presurgery and early postsurgery performance related to speech and language outcomes at 3 years o...