| Alkyl-substituted methoxysilanes enhance the activity and stability of D: -amino acid oxidase encapsulated in biomimetic silica. | |
| | |
MedLine Citation:
|
PMID: 22488440 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D: -amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PAA)-or R5 peptide-catalyzed silicic encapsulation, the RtDAO activity increased with the degree of substitution and the molar ratio up to 15 % of methyl-substituted silanes added. In the presence of 15 mol% trimethylmethoxysilane, the specific activities of encapsulated RtDAO catalyzed by PAA and R5 increased by 1.4- and 4.8-fold, respectively. For PAA-catalyzed encapsulation, a 2.4-fold increase occurred with 30 mol% n-propyltrimethoxysilane; this modification increased the T (m) value by 10 °C and gave a threefold longer half-life in the presence of 10 mM H(2)O(2) as compared to the encapsulation using tetramethoxysilane only. |
| | |
Authors:
|
I-Ching Kuan; Chiao-An Chuang; Shiow-Ling Lee; Chi-Yang Yu |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-10 |
Journal Detail:
|
Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Bioengineering, Tatung University, 40 Chungshan N. Rd. Sec. 3, Taipei, 10452, Taiwan, iching@ttu.edu.tw. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development of broad-spectrum insect-resistant tobacco by expression of synthetic cry1Ac and cry2Ab ...
Next Document: SK-HEP cells and lentiviral vector for production of human recombinant factor VIII.