Document Detail


Alkaloid production in Vernonia cinerea: Callus, cell suspension and root cultures.
MedLine Citation:
PMID:  17582823     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fast-growing callus, cell suspension and root cultures of Vernonia cinerea, a medicinal plant, were analyzed for the presence of alkaloids. Callus and root cultures were established from young leaf explants in Murashige and Skoog (MS) basal media supplemented with combinations of auxins and cytokinins, whereas cell suspension cultures were established from callus cultures. Maximum biomass of callus, cell suspension and root cultures were obtained in the medium supplemented with 1 mg/L alpha-naphthaleneacetic acid (NAA) and 5 mg/L benzylaminopurine (BA), 1.0 mg/L NAA and 0.1 mg/L BA and 1.5 mg/L NAA, respectively. The 5-week-old callus cultures resulted in maximum biomass and alkaloid contents (750 microg/g). Cell suspension growth and alkaloid contents were maximal in 20-day-old cultures and alkaloid contents were 1.15 mg/g. A 0.2-g sample of root tissue regenerated in semi-solid medium upon transfer to liquid MS medium containing 1.5 mg/L NAA regenerated a maximum increase in biomass of 6.3-fold over a period of 5 weeks. The highest root growth and alkaloid contents of 2 mg/g dry weight were obtained in 5-week-old cultures. Maximum alkaloid contents were obtained in root cultures in vitro compared to all others including the alkaloid content of in vivo obtained with aerial parts and roots (800 microg/g and 1.2 mg/g dry weight, respectively) of V. cinerea.
Authors:
Priti Maheshwari; Bharti Songara; Shailesh Kumar; Prachi Jain; Kamini Srivastava; Anil Kumar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology journal     Volume:  2     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-16     Completed Date:  2007-09-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1026-32     Citation Subset:  IM    
Affiliation:
School of Biotechnology, Devi Ahilya University, Indore, India.
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MeSH Terms
Descriptor/Qualifier:
Agriculture / methods*
Alkaloids / metabolism*
Cell Culture Techniques / methods*
Cells, Cultured
Plant Roots / metabolism*
Vernonia / metabolism*
Chemical
Reg. No./Substance:
0/Alkaloids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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