Document Detail


Alkaline phosphatase and peptidase activities in Caco-2 cells: differential response to triiodothyronine.
MedLine Citation:
PMID:  7881629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caco-2 cell human colon adenocarcinoma cell line was used to study the hormonal regulation of small intestinal epithelial cell differentiation. We had previously shown that insulin-transferrin-selenium and triiodothyronine (5 x 10(-8) M)-supplemented medium can best replace serum after 2 days of culture for both the maintenance and differentiation of Caco-2 cells. The present study demonstrates that precoating petri dishes with complete serum allows the growth and differentiation of Caco-2 cells seeded directly in serum-free medium. On the other hand, precoating with dialyzed serum inhibits alkaline phosphatase and dipeptidyl-dipeptidase IV activities by more than 50%. The results obtained with complete serum-precoated culture plates indicate that there is no synergy between insulin and triiodothyronine because cells maintained in transferrin-selenium and triiodothyronine-supplemented medium, with or without insulin, express comparable enzyme activities. Moreover, large increases in alkaline phosphatase and dipeptidyl-dipeptidase IV activities were observed when triiodothyronine was added to the culture medium by the time confluency was reached. In contrast, gamma-glutamyltransferase was lowered to a greater extent when triiodothyronine was present from the beginning of culture. These findings show that triiodothyronine preferentially stimulates alkaline phosphatase and dipeptidyl-dipeptidase IV activities during the differentiation period whereas it selectively inhibits gamma-glutamyltransferase during the proliferation phase. Triiodothyronine acts in a dose-dependent manner.
Authors:
C Jumarie; C Malo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  30A     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-04-11     Completed Date:  1995-04-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  753-60     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine, University of Montréal, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / enzymology*,  pathology
Alkaline Phosphatase / metabolism*
Antigens, CD26 / metabolism*
Cell Communication / drug effects,  physiology
Cell Division / drug effects,  physiology
Colonic Neoplasms / enzymology*,  pathology
Culture Media, Serum-Free
Dose-Response Relationship, Drug
Enzyme Activation / drug effects
Humans
Insulin / pharmacology
Triiodothyronine / pharmacology*
Tumor Cells, Cultured
gamma-Glutamyltransferase / antagonists & inhibitors,  metabolism*
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 11061-68-0/Insulin; 6893-02-3/Triiodothyronine; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.4.14.5/Antigens, CD26

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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