| Alkaline phosphatase expression in human chorionic villi. | |
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MedLine Citation:
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PMID: 3569636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The physicochemical properties and electrophoretic mobility of different isoforms of alkaline phosphatase were studied in chorionic villi. Based on selective inactivation and inhibition studies (thermal stability, inactivation by urea, EDTA and L(+)ascorbic acid and L-amino acid inhibition), evidence was obtained for the existence of two distinct types of alkaline phosphatase in trophoblast cells. One type is peculiar to chorionic villi while the other is also found in term placenta. Both show two isoforms. These two isoforms were observed with polyacrylamide gel electrophoresis, carried out at pH 6.0 and 9.5. It is suggested that the qualitative and quantitative methods of alkaline phosphatase analysis could be used for first trimester fetal diagnosis of severe infantile hypophosphatasia and for understanding genetic control during early fetal development. |
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Authors:
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G Novelli; F Mannello; E V Cosmi; S Biagioni; B Dallapiccola |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell biology Volume: 55 ISSN: 0304-3568 ISO Abbreviation: Exp. Cell Biol. Publication Date: 1987 |
Date Detail:
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Created Date: 1987-06-08 Completed Date: 1987-06-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7701827 Medline TA: Exp Cell Biol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 34-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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antagonists & inhibitors,
metabolism* Amino Acids / pharmacology Chorionic Villi / enzymology* Female Humans Isoenzymes / metabolism Kinetics |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Isoenzymes; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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