Document Detail

Alkaline lability of fluorescent photoproducts produced in ultraviolet light-irradiated DNA.
MedLine Citation:
PMID:  7142164     Owner:  NLM     Status:  MEDLINE    
Ultraviolet light induces alkaline labile lesions in DNA. These lesions occur at the bipyrimidine sites T-C, C-C, and T-T, and do not result from the formation of pyrimidine cyclobutane dimers. To examine the chemical nature of the alkaline labile lesions, pyrimidine dinucleotides (2'-deoxythymidylyl-(3' leads to 5')-2'-deoxycytidine, 2'-deoxythymidylyl-(3' leads to 5')-2'-deoxythymidine, 2'-deoxycytidylyl-(3' leads to 5')-2-deoxycytidine, and 2'-deoxycytidylyl-(3' leads to 5')-2'-deoxythymidine) were used as a model system. Ultraviolet light irradiation of all four dinucleotides resulted in the formation of substantial quantities (relative to cyclobutane pyrimidine dimers) of products that were red-shifted in UV absorbance and fluorescent. These products are identified as precursors to the 6-4'-[pyrimidin-2'-one]-pyrimidine class of products that have been previously shown to occur in UV-irradiated DNA. The fluorescent products were found to be alkaline labile; the products contained a 3'-phosphate end group after alkali treatment. Two of the fluorescent products have been found in enzymatic digests of high dose UV-irradiated salmon sperm DNA, the T-C and T-T UV-induced products. The relative rates of formation of these products in 32P-radiolabeled dinucleotides were measured. We conclude that the alkaline labile lesions observed in DNA at the bipyrimidine sites are the same as those that yield the 6-4 products upon acid hydrolysis of DNA. The mechanisms for the formation and the consequences of DNA structure for these lesions as well as the possible biological significance of this class of UV damage are discussed.
W A Franklin; K M Lo; W A Haseltine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-01-07     Completed Date:  1983-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13535-43     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Chromatography, High Pressure Liquid
DNA / radiation effects*
Dose-Response Relationship, Radiation
Drug Stability
Hydrogen-Ion Concentration
Ultraviolet Rays*
Grant Support
Reg. No./Substance:

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