| Alk-1-enylacyl, alkylacyl, and diacyl subclasses of native ethanolamine and choline glycerophospholipids can be quantified directly by phosphorus-31 NMR in solution. | |
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MedLine Citation:
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PMID: 8934452 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We show that phosphorus-31 nuclear magnetic resonance spectroscopy can be used to distinguish and to quantify the alk-1-enylacyl, alkylacyl, and diacyl glycerophosphoethanolamine (GPE) subclasses, and the respective glycerophosphocholine (GPC) subclasses, in their native form without prior degradation or derivatization, provided the phospholipids are observed in the nonaggregated state. Monomeric phospholipid distribution is ascertained by recording the spectra, after removal of metal ions, on CDCl3/CD3OD/D2O (50:50:15, by vol) solutions. The utility of this approach is exemplified for the ethanolamine glycerophospholipids (EPL) from bovine brain and the choline glycerophospholipids (CPL) from bovine heart. Sharp and well-resolved resonances are obtained for alkylacylGPE (+0.395 ppm; re 1% H3PO4), alkenylacylGPE (+0.353 ppm), and diacylGPE (+0.315 ppm), and for alkylacylGPC (-0.383 ppm), alkenyl-acylGPC (-0.436 ppm) and diacylGPC (-0.451 ppm). Integrated peak areas are shown to closely correlate with dose. Accurate quantitation of EPL and CPL subclasses at submicromolar levels can further be facilitated by use of synthetic dialkylGPE (+0.602 ppm) and dialkylGPC (-0.196 ppm) as internal standards. The method is simple, rapid, sensitive and reproducible, and permits the complete resolution and direct quantitation of all ethanolamine and choline glycerophospholipid subclasses quite independent of fatty chain length and degree of unsaturation. |
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Authors:
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B Malewicz; W J Baumann |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Lipids Volume: 31 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-03-28 Completed Date: 1997-03-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1189-95 Citation Subset: IM |
Affiliation:
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Membrane Chemistry and Biology Section, Hormel Institute, University of Minnesota, Austin 55912, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry Cattle Magnetic Resonance Spectroscopy / methods* Molecular Structure Myocardium / chemistry Phosphatidylcholines / analysis*, chemistry*, classification Phosphatidylethanolamines / analysis*, chemistry*, classification Phosphorus / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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RR04654/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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