Document Detail

Aligned Hybrid Silk Scaffold for Enhanced Differentiation of Mesenchymal Stem Cells into Ligament Fibroblasts.
MedLine Citation:
PMID:  21385018     Owner:  NLM     Status:  Publisher    
The concept of contact guidance utilizes the phenomenon of anchorage dependence of cells on the topography of seeded surfaces. It has been shown in previous studies that cells were guided to align along the topographical alignment of the seeding substrate and produced enhanced amounts of oriented extracellular matrix (ECM). In this study, we aimed to apply this concept to a three-dimensional full silk fibroin (SF) hybrid scaffold system, which comprised of knitted SF and aligned SF electrospun fibers (SFEFs), for ligament tissue engineering applications. Specifically, knitted SF, which contributed to the mechanical robustness of the system, was integrated with highly aligned SFEF (AL-SFEF) mesh, which acted as the initial ECM to provide environmental cues for positive cellular response. Mesenchymal stem cells (MSCs) seeded on the aligned hybrid scaffolds were shown to be proliferative and aligned along the integrated AL-SFEF, forming oriented spindle-shaped morphology and produced an aligned ECM network. Expression and production of ligament-related proteins were also increased as compared to hybrid SF scaffolds with randomly arranged SFEFs (RD-SFEF), indicating differentiative cues for ligament fibroblasts present in the aligned hybrid SF scaffolds. Consequently, the tensile properties of cultured aligned constructs were significantly improved and superior to the counterpart with RD-SFEF. These results thus showed that the aligned hybrid scaffold system was promising for enhancing cell proliferation, differentiation and function for ligament tissue engineering applications.
Thomas Kok Hiong Teh; Siew Lok Toh; James Cho Hong Goh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-8
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  -     ISSN:  1937-3392     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
National University of Singapore, Division of Bioengineering, Singapore, Singapore;
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