Document Detail


Alginate acetylation influences initial surface colonization by mucoid Pseudomonas aeruginosa.
MedLine Citation:
PMID:  15881834     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mucoid strains of Pseudomonas aeruginosa overproduce the exopolysaccharide alginate, which is substituted with O-acetyl groups. Under non-growing conditions in phosphate buffer, a mucoid clinical strain formed microcolonies on steel surfaces, while an acetylation-defective mutant was unable to form cell clusters. Enzymatic degradation of alginate by alginate lyase prevented microcolony formation of the mucoid parent strain. In a continuous-culture flow-cell system, using gluconate minimal medium, the mucoid strain with acetylated alginate formed microcolonies and grew into heterogenous biofilms, whereas the acetylation-defective mutant produced a thinner and more homogeneous biofilm. A lowered viscosity of extracellular material from the acetylation-defective mutant indicated a weakening of exopolymer interactions by loss of acetyl groups. These results suggest that acetyl substituents are necessary for the function of high-molecular-mass alginate to mediate cell aggregation into microcolonies in the early stages of biofilm development by mucoid P. aeruginosa, thereby determining the architecture of the mature biofilm.
Authors:
Petra Tielen; Martin Strathmann; Karl-Erich Jaeger; Hans-Curt Flemming; Jost Wingender
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiological research     Volume:  160     ISSN:  0944-5013     ISO Abbreviation:  Microbiol. Res.     Publication Date:  2005  
Date Detail:
Created Date:  2005-05-10     Completed Date:  2005-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9437794     Medline TA:  Microbiol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  165-76     Citation Subset:  IM    
Affiliation:
Department of Aquatic Microbiology, Biofilm Centre, University of Duisburg-Essen, Geibelstrasse 41, 47057 Duisburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Alginates / metabolism*
Bacterial Adhesion / physiology*
Biofilms / growth & development*
Culture Media
Glucuronic Acid / metabolism*
Hexuronic Acids / metabolism*
Humans
Mutation
Polysaccharide-Lyases / genetics,  metabolism
Polysaccharides, Bacterial / metabolism
Pseudomonas aeruginosa / enzymology,  genetics,  growth & development*,  physiology
Chemical
Reg. No./Substance:
0/Alginates; 0/Culture Media; 0/Hexuronic Acids; 0/Polysaccharides, Bacterial; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.3/poly(beta-D-mannuronate) lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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