| Alginate acetylation influences initial surface colonization by mucoid Pseudomonas aeruginosa. | |
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MedLine Citation:
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PMID: 15881834 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mucoid strains of Pseudomonas aeruginosa overproduce the exopolysaccharide alginate, which is substituted with O-acetyl groups. Under non-growing conditions in phosphate buffer, a mucoid clinical strain formed microcolonies on steel surfaces, while an acetylation-defective mutant was unable to form cell clusters. Enzymatic degradation of alginate by alginate lyase prevented microcolony formation of the mucoid parent strain. In a continuous-culture flow-cell system, using gluconate minimal medium, the mucoid strain with acetylated alginate formed microcolonies and grew into heterogenous biofilms, whereas the acetylation-defective mutant produced a thinner and more homogeneous biofilm. A lowered viscosity of extracellular material from the acetylation-defective mutant indicated a weakening of exopolymer interactions by loss of acetyl groups. These results suggest that acetyl substituents are necessary for the function of high-molecular-mass alginate to mediate cell aggregation into microcolonies in the early stages of biofilm development by mucoid P. aeruginosa, thereby determining the architecture of the mature biofilm. |
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Authors:
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Petra Tielen; Martin Strathmann; Karl-Erich Jaeger; Hans-Curt Flemming; Jost Wingender |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiological research Volume: 160 ISSN: 0944-5013 ISO Abbreviation: Microbiol. Res. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-05-10 Completed Date: 2005-07-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9437794 Medline TA: Microbiol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 165-76 Citation Subset: IM |
Affiliation:
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Department of Aquatic Microbiology, Biofilm Centre, University of Duisburg-Essen, Geibelstrasse 41, 47057 Duisburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Alginates / metabolism* Bacterial Adhesion / physiology* Biofilms / growth & development* Culture Media Glucuronic Acid / metabolism* Hexuronic Acids / metabolism* Humans Mutation Polysaccharide-Lyases / genetics, metabolism Polysaccharides, Bacterial / metabolism Pseudomonas aeruginosa / enzymology, genetics, growth & development*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 0/Culture Media; 0/Hexuronic Acids; 0/Polysaccharides, Bacterial; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.3/poly(beta-D-mannuronate) lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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