Document Detail

Algae permeability to Me(2)SO from -3 to 23 degrees C.
MedLine Citation:
PMID:  11748937     Owner:  NLM     Status:  MEDLINE    
Biphasic transport of water and dimethyl sulfoxide (Me(2)SO), a common cryoprotective agent (CPA), in algal cells was induced and measured on a cryoperfusion stage. A two-step experimental protocol provided data for the volumetric response of Chlorococcum (C.) texanum to impermeable and permeable solutes. First, the cells were exposed to a 500-mOsm sucrose solution, causing immediate shrinkage of the cell to a minimum equilibrium volume. Then an isoosmotic 200-mOsm/300-mOsm CPA/sucrose solution was introduced to the cells, resulting in increased cell volume to a new equilibrium state. Experiments were conducted at temperatures between -3 and 23 degrees C. Cell volumes were measured off-line by computer analysis of video images. A network thermodynamic model was fit to the transient volume data to determine permeabilities of C. texanum to water and Me(2)SO over the full temperature range, and results were calculated with two numeric methods. Biphasic transport was found to be slower at colder temperatures, with water entering the cell faster than Me(2)SO. Experimental results were also compared with data from similar experiments using methanol (MeOH) as the CPA. MeOH influx was calculated to be a magnitude larger than that of water. Additionally, MeOH permeability was at least three orders of magnitude greater than Me(2)SO permeability, and the difference in these solute permeabilities increased as temperature decreased.
J Y Tanaka; J R Walsh; K R Diller; J J Brand; S J Aggarwal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  42     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2002-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  286-300     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Elsevier Science.
Department of Biomedical Engineering, University of Texas at Austin, 78712, USA.
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MeSH Terms
Algae, Green / cytology,  metabolism*
Biological Transport, Active
Cryopreservation / methods
Cryoprotective Agents / pharmacokinetics*
Dimethyl Sulfoxide / pharmacokinetics*
Microscopy / instrumentation
Models, Biological
Water / metabolism
Reg. No./Substance:
0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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